西班牙Certest乙型流感病毒重組核蛋白
廣州健侖生物科技有限公司
廣州健侖長期供應各種生物原料,主要代理品牌:西班牙Certest。
Certest Biotec,S.L. 是一家獨立的生物技術公司,致力于人類臨床領域的IVD診斷產品的開發和制造。快速檢測是基于快速、準確和易于操作的診斷產品。 此外,Cerstest還有多種實時PCR產品,可以用于醫院臨床、實驗室科研等。
Certest公司于2002年在薩拉戈薩成立,是一家創新技術型公司。公司的發展是基于新產品的開發、市場空間和機遇的探索,十幾年來一直以高度專業化,以客戶為導向,不斷得創新,優化產品,專業知識,取得廣大客戶的信任和支持。Certest的質量體系已通過ISO 13485認證。
主要產品包括各種生物單克隆抗原抗體、重組蛋白。
輪狀病毒單克隆抗體、腺病毒抗體、星狀病毒單克隆抗體、諾如病毒單克隆抗體、幽門螺旋桿菌抗體、隱球菌抗體、腸道病毒抗體、賈第鞭毛蟲抗體、彎曲桿菌抗體、阿米巴原蟲抗體、呼吸道合胞病毒單抗等等。
西班牙Certest乙型流感病毒重組核蛋白
我司還提供其它進口或國產試劑盒:登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。
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西班牙Certest公司簡介
Certest Biotec, S.L. is an independent biotechnology company devoted to the development and manufacturing of IVD diagnostic products in human clinical field. Our Rapid Test are based in a fast, reliable and easy-interpretation process. In addition, VIASURE contains a wide range of products for Real Time PCR that allow to identify the causal pathogens of the infectious diseases in physicians’ offices, labs and hospitals.
The company was established in Zaragoza in 2002 as an innovative and technology-based company. The company’s growth is based on the development of new products and the exploration for new market niches and opportunities. The key to understand its success is the way to understand its organization: highly specialised and customer-oriented based.
Mission, Vision, Values and Human Team
Mission
To develop, produce and market a wide range of innovative solutions and quality in vitro diagnostics for the detection of viruses, bacteria and parasites.
Vision
Being the international leader company in research and development for in vitro diagnostic solutions, increasing our distribution network through added value creation and company-customer trust.
Values
Customer orientation · Ethical behaviour · Teamwork · Continuous research and innovation ·Trained and motivated team · Quality and creativity in all processes
Human team
The multi-skilled team is a dedicated and experience professional group responsible for dealing with all the different company activities in an international environment.
Commitment to Innovation, Export and High-Technology.
Certest’s Quality System has been certified with ISO 13485.
One of the main objectives of Certest is to achieve customer satisfaction. For this, the management of the company ensures that customer requirements are determined and reviewed, identifying their needs and expectations, and translating them into requirements, all in order to maintain customer satisfaction.
See/Download the Certest Biotec ISO Standard Certification Certest Policy is consistent with its primary purpose, that of getting to all its products and services, quality and reliability, meeting the requirements and maintaining the effectiveness of the management system.
“Comparative study of two diagnostic tests for detection of RNA Zika Virus (ZIKV) in clinical samples”
“Comparison of Real-Time PCR test with the routine diagnosis technique to detect enteric pathogenic protozoa”
“Diagnostic of the Enteric Virus infection by Real-Time PCR in stool samples”
“Clinical performance evaluation of lyophilized VIASURE Real Time PCR Detection Kits for pathogen detection by participation in External Quality Assessment (EQA) programs”
“Detection and differentiation of Influenza A Virus, Influenza B Virus and H1N1 (pdm09) variant through an stabilized Real Time PCR Mix”
“Comparison of different molecular methods for the detection of non-influenza respiratory virus”virus”
【西班牙Certest生物原料】
貨號 | 產品名稱 | 規格 | 英文名稱 |
MT-16R15 | 輪狀病毒單克隆抗體(克隆R15) | x1mg | Anti-Rotavirus Mab (clone R15) |
MT-16R15s1 | 輪狀病毒單克隆抗體(克隆R15) | x100μg | Anti-Rotavirus Mab (clone R15) |
MT-18A15 | 腺病毒抗體(克隆A15) | x1mg | Anti-Adenovirus Mab (clone A15) |
MT-18A15s1 | 腺病毒抗體(克隆A15) | x100μg | Anti-Adenovirus Mab (clone A15) |
MT-18AT18 | 星狀病毒單克隆抗體(克隆AT18) | x1mg | Anti-Astrovirus Mab (clone AT18) |
MT-18AT18s1 | 星狀病毒單克隆抗體(克隆AT18) | x100μg | Anti-Astrovirus Mab (clone AT18) |
MT-18AT8 | 星狀病毒單克隆抗體(克隆AT8) | x1mg | Anti-Astrovirus Mab (clone AT8) |
MT-18AT8s1 | 星狀病毒單克隆抗體(克隆AT8) | x100μg | Anti-Astrovirus Mab (clone AT8) |
MT-18NG28 | 諾如病毒GI單克隆抗體(克隆NG28) | x1mg | Anti-Norovirus GI Mab (clone NG28) |
MT-18NG28sl | 諾如病毒GI單克隆抗體(克隆NG28) | x100μg | Anti-Norovirus GI Mab (clone NG28) |
MT-18NP8 | 諾如病毒GII單克隆抗體(克隆NP8) | x1mg | Anti-Norovirus GII Mab (clone NP8) |
MT-18NP8sl | 諾如病毒GII單克隆抗體(克隆NP8) | x100μg | Anti-Norovirus GII Mab (clone NP8) |
MT-18EV5 | 腸道病毒抗體(克隆EV5) | x1mg | Anti-Enterovirus Mab (clone EV5) |
MT-18EV5sl | 腸道病毒抗體(克隆EV5) | x100μg | Anti-Enterovirus Mab (clone EV5) |
MT-16P2 | 幽門螺旋桿菌抗體(克隆P2)HP抗體 | x1mg | Anti-H. pylori Mab (clone P2) |
MT-16P2sl | 幽門螺旋桿菌抗體(克隆P2)HP抗體 | x100μg | Anti-H. pylori Mab (clone P2) |
MT-16GD10 | 艱難梭菌抗體(克隆GD10) | x1mg | Anti-GDH Mab (clone GD10) |
MT-16GD10sl | 艱難梭菌抗體(克隆GD10) | x100μg | Anti-GDH Mab (clone GD10) |
MT-18TA5 | 艱難梭菌毒素A抗(克隆TA5) | x1mg | Anti-CD Toxin A Mab (clone TA5) |
MT-18TA5sl | 艱難梭菌毒素A抗(克隆TA5) | x100μg | Anti-CD Toxin A Mab (clone TA5) |
MT-18TA7 | 艱難梭菌毒素A抗(克隆TA7) | x1mg | Anti-CD Toxin A Mab (clone TA7) |
MT-18TA7sl | 艱難梭菌毒素A抗(克隆TA7) | x100μg | Anti-CD Toxin A Mab (clone TA7) |
MT-18TB41 | 艱難梭菌毒素B抗(克隆TB41) | x1mg | Anti-CD Toxin B Mab (clone TB41) |
MT-18TB41sl | 艱難梭菌毒素B抗(克隆TB41) | x100μg | Anti-CD Toxin B Mab (clone TB41) |
MT-18TB48 | 艱難梭菌毒素B抗(克隆TB48) | x1mg | Anti-CD Toxin B Mab (clone TB48) |
MT-18TB48sl | 艱難梭菌毒素B抗(克隆TB48) | x100μg | Anti-CD Toxin B Mab (clone TB48) |
MT-18E10 | 大腸桿菌O157抗體(克隆E10) | x1mg | Anti-E. coli O157 Mab (clone E10) |
MT-18E10sl | 大腸桿菌O157抗體(克隆E10) | x100μg | Anti-E. coli O157 Mab (clone E10) |
MT-16CA29 | 彎曲桿菌抗體(克隆ECA29) | x1mg | Anti-Campylobacter Mab (clone CA29) |
MT-16CA29sl | 彎曲桿菌抗體(克隆ECA29) | x100μg | Anti-Campylobacter Mab (clone CA29) |
MT-18K31 | 隱球菌抗體(克隆K31) | x1mg | Anti-Crypto Mab (clone K31) |
MT-18K31sl | 隱球菌抗體(克隆K31) | x100μg | Anti-Crypto Mab (clone K31) |
MT-16G18 | 賈第鞭毛蟲抗體(克隆G18) | x1mg | Anti-Giardia Mab trophozoite protein (clone G18) |
MT-16G18sl | 賈第鞭毛蟲抗體(克隆G18) | x100μg | Anti-Giardia Mab trophozoite protein (clone G18) |
MT-16G22 | 賈第鞭毛蟲抗體(克隆G22) | x1mg | Anti-Giardia Mab trophozoite protein (clone G22) |
MT-16G22sl | 賈第鞭毛蟲抗體(克隆G22) | x100μg | Anti-Giardia Mab trophozoite protein (clone G22) |
MT-18EH30 | 阿米巴原蟲抗體(克隆H30) | x1mg | Anti-Entamoeba Mab (clone EH30) |
MT-18EH30sl | 阿米巴原蟲抗體(克隆H30) | x100μg | Anti-Entamoeba Mab (clone EH30) |
MT-16CP14 | 鈣結合蛋白單克隆抗體(克隆CP14) | x1mg | Anti-Calprotectin Mab (clone CP14) |
MT-16CP14sl | 鈣結合蛋白單克隆抗體(克隆CP14) | x100μg | Anti-Calprotectin Mab (clone CP14) |
MT-16F22 | 血紅蛋白單抗(克隆F22) | x1mg | Anti-Haemoglobin Mab (clone F22) |
MT-16F22sl | 血紅蛋白單抗(克隆F22) | x100μg | Anti-Haemoglobin Mab (clone F22) |
MT-16LC16 | 乳鐵蛋白單抗(克隆LC16) | x1mg | Anti-Lactoferrin Mab (clone LC16) |
MT-16LC16sl | 乳鐵蛋白單抗(克隆LC16) | x100μg | Anti-Lactoferrin Mab (clone LC16) |
MT-16LC4 | 乳鐵蛋白單抗(克隆LC4) | x1mg | Anti-Lactoferrin Mab (clone LC4) |
MT-16LC4sl | 乳鐵蛋白單抗(克隆LC4) | x100μg | Anti-Lactoferrin Mab (clone LC4) |
MT-26VP6 | 輪狀病毒VP6重組蛋白 | x1mg | Rotavirus VP6 recombinant protein |
MT-26VP6 | 輪狀病毒VP6重組蛋白 | x100μg | Rotavirus VP6 recombinant protein |
MT-25HEX | 腺病毒HEXON重組蛋白 | x1mg | Adenovirus HEXON recombinant protein |
MT-25HEXsl | 腺病毒HEXON重組蛋白 | x100μg | Adenovirus HEXON recombinant protein |
MT-25AST | 星狀病毒衣殼重組蛋白 | x1mg | Astrovirus capsid recombinant protein |
MT-25ASTsl | 星狀病毒衣殼重組蛋白 | x100μg | Astrovirus capsid recombinant protein |
MT-25NGI1 | 諾如病毒GI.1重組P結構域 | x1mg | Norovirus GI.1 recombinant P domain |
MT-25NGI1sl | 諾如病毒GI.1重組P結構域 | x100μg | Norovirus GI.1 recombinant P domain |
MT-25NGI3 | 諾如病毒GI.3重組P結構域 | x1mg | Norovirus GI.3 recombinant P domain |
MT-25NGI3sl | 諾如病毒GI.3重組P結構域 | x100μg | Norovirus GI.3 recombinant P domain |
MT-25NGII10 | 諾如病毒GII.10重組P結構域 | x1mg | Norovirus GII.10 recombinant P domain |
MT-25NGII10sl | 諾如病毒GII.10重組P結構域 | x100μg | Norovirus GII.10 recombinant P domain |
MT-25NGII17 | 諾如病毒GII.17重組P結構域 | x1mg | Norovirus GII.17 recombinant P domain |
MT-25NGII17sl | 諾如病毒GII.17重組P結構域 | x100μg | Norovirus GII.17 recombinant P domain |
MT-25NGII14 | 諾如病毒GII.4重組P結構域 | x1mg | Norovirus GII.4 recombinant P domain |
MT-25NGII14sl | 諾如病毒GII.4重組P結構域 | x100μg | Norovirus GII.4 recombinant P domain |
MT-25ETV | 腸道病毒VP1重組蛋白 | x1mg | Enterovirus VP1 recombinant protein |
MT-25ETVs1 | 腸道病毒VP1重組蛋白 | x100μg | Enterovirus VP1 recombinant protein |
MT-25PCH | 幽門螺桿菌重組外膜蛋白 | x1mg | H. pylori recombinant outer membrane protein |
MT-25PCHs1 | 幽門螺桿菌重組外膜蛋白 | x100μg | H. pylori recombinant outer membrane protein |
MT-25GDH | 艱難梭菌GDH重組蛋白 | x1mg | Clostridium difficile GDH recombinant protein |
MT-25GDHsl | 艱難梭菌GDH重組蛋白 | x100μg | Clostridium difficile GDH recombinant protein |
MT-24TXA | 艱難梭菌毒素A重組蛋白(無毒性片段) | x1mg | C. difficile Toxin A recombinant protein (fragment without toxic activity) |
MT-24TXAsl | 艱難梭菌毒素A重組蛋白(無毒性片段) | x100μg | C. difficile Toxin A recombinant protein (fragment without toxic activity) |
MT-24TXB | 艱難梭菌毒素B重組蛋白(無毒性片段) | x1mg | C. difficile Toxin B recombinant protein (fragment without toxic activity) |
MT-24TXBsl | 艱難梭菌毒素B重組蛋白(無毒性片段) | x100μg | C. difficile Toxin B recombinant protein (fragment without toxic activity) |
MT-25STX | 大腸桿菌O157 VT1重組蛋白 | x1mg | E. coli O157 VT1 recombinant protein |
MT-25STXsl | 大腸桿菌O157 VT1重組蛋白 | x100μg | E. coli O157 VT1 recombinant protein |
MT-25VT2 | 大腸桿菌O157 VT2重組蛋白 | x1mg | E. coli O157 VT2 recombinant protein |
MT-25VT2sl | 大腸桿菌O157 VT2重組蛋白 | x100μg | E. coli O157 VT2 recombinant protein |
MT-25CCP | 彎曲桿菌重組外膜蛋白 | x1mg | Campylobacter coli recombinant outer membrane protein |
MT-25CCPsl | 彎曲桿菌重組外膜蛋白 | x100μg | Campylobacter coli recombinant outer membrane protein |
MT-25CEP | 空腸彎曲桿菌重組外膜蛋白 | x1mg | Campylobacter jejuni recombinant outer membrane protein |
MT-25CEPsl | 空腸彎曲桿菌重組外膜蛋白 | x100μg | Campylobacter jejuni recombinant outer membrane protein |
MT-25A1G | 賈第蟲腸道滋養體重組蛋白 | x1mg | Giardia intestinalis trophozoite recombinant protein |
MT-25A1Gsl | 賈第蟲腸道滋養體重組蛋白 | x100μg | Giardia intestinalis trophozoite recombinant protein |
MT-25GCP | 賈第蟲腸囊菌重組蛋白 | x1mg | Giardia intestinalis cyst recombinant protein |
MT-25GCPsl | 賈第蟲腸囊菌重組蛋白 | x100μg | Giardia intestinalis cyst recombinant protein |
MT-25EDP | 內阿米巴重組蛋白 | x1mg | Entamoeba dispar recombinant protein |
MT-25EDPsl | 內阿米巴重組蛋白 | x100μg | Entamoeba dispar recombinant protein |
MT-25EHP | 溶組織內阿米巴重組蛋白 | x1mg | Entamoeba histolytica recombinant protein |
MT-25EHPsl | 溶組織內阿米巴重組蛋白 | x100μg | Entamoeba histolytica recombinant protein |
MT-25HCP | 人類鈣衛蛋白重組蛋白 | x1mg | Human Calprotectin recombinant protein |
MT-25HCPs1 | 人類鈣衛蛋白重組蛋白 | x100μg | Human Calprotectin recombinant protein |
MT-31NGA | 諾如病毒GI.1重組VLP | x1mg | Norovirus GI.1 recombinant VLP |
MT-31NGAsl | 諾如病毒GI.1重組VLP | x100μg | Norovirus GI.1 recombinant VLP |
MT-31NPA | 諾如病毒GII.4重組VLP | x1mg | Norovirus GII.4 recombinant VLP |
MT-31NPAsl | 諾如病毒GII.4重組VLP | x100μg | Norovirus GII.4 recombinant VLP |
MT-28PECU | 滅活的幽門螺桿菌抗原(天然提取物) | x1mg | Inactivated H. pylori antigen (native extract) |
MT-28PECUsl | 滅活的幽門螺桿菌抗原(天然提取物) | x100μg | Inactivated H. pylori antigen (native extract) |
MT-28EC7U | 滅活的大腸桿菌O157抗原(天然提取物) | x1mg | Inactivated E. coli O157 antigen (native extract) |
MT-28EC7Usl | 滅活的大腸桿菌O157抗原(天然提取物) | x100μg | Inactivated E. coli O157 antigen (native extract) |
MT-28CCU | 滅活的大腸桿菌抗原(天然提取物) | x1mg | Inactivated Campylobacter coli antigen (native extract) |
MT-28CCUsl | 滅活的大腸桿菌抗原(天然提取物) | x100μg | Inactivated Campylobacter coli antigen (native extract) |
MT-28CJU | 滅活的空腸彎曲桿菌抗原(天然提取物) | x1mg | Inactivated Campylobacter jejuni antigen (native extract) |
MT-28CJUsl | 滅活的空腸彎曲桿菌抗原(天然提取物) | x100μg | Inactivated Campylobacter jejuni antigen (native extract) |
MT-28SEU | 滅活腸炎沙門氏菌抗原(天然提取物) | x1mg | Inactivated Salmonella enteritidis antigen (native extract) |
MT-28SEUs1 | 滅活腸炎沙門氏菌抗原(天然提取物) | x100μg | Inactivated Salmonella enteritidis antigen (native extract) |
MT-28SPAU | 滅活沙門氏菌副傷寒A抗原(天然提取物) | x1mg | Inactivated Salmonella paratyphi A antigen (native extract) |
MT-28SPAUsl | 滅活沙門氏菌副傷寒A抗原(天然提取物) | x100μg | Inactivated Salmonella paratyphi A antigen (native extract) |
MT-28SPBU | 滅活沙門氏菌副傷寒B抗原(天然提取物) | x1mg | Inactivated Salmonella paratyphi B antigen (native extract) |
MT-28SPBUsl | 滅活沙門氏菌副傷寒B抗原(天然提取物) | x100μg | Inactivated Salmonella paratyphi B antigen (native extract) |
MT-28STMU | 滅活的鼠傷寒沙門氏菌抗原(天然提取物) | x1mg | Inactivated Salmonella typhimurium antigen (native extract) |
MT-28STMUsl | 滅活的鼠傷寒沙門氏菌抗原(天然提取物) | x100μg | Inactivated Salmonella typhimurium antigen (native extract) |
MT-28STU | 滅活傷寒沙門氏菌抗原(天然提取物) | x1mg | Inactivated Salmonella typhi antigen (native extract) |
MT-28STUsl | 滅活傷寒沙門氏菌抗原(天然提取物) | x100μg | Inactivated Salmonella typhi antigen (native extract) |
MT-28LMU | 滅活的單核細胞增生李斯特菌抗原(天然提取物) | x1mg | Inactivated Listeria monocytogenes antigen (native extract) |
MT-28LMUsl | 滅活的單核細胞增生李斯特菌抗原(天然提取物) | x100μg | Inactivated Listeria monocytogenes antigen (native extract) |
MT-28YE3U | 滅活小腸結腸炎耶爾森氏菌O:3抗原(天然提取物) | x1mg | Inactivated Yersinia enterocolitica O:3 antigen (native extract) |
MT-28YE3Usl | 滅活小腸結腸炎耶爾森氏菌O:3抗原(天然提取物) | x100μg | Inactivated Yersinia enterocolitica O:3 antigen (native extract) |
MT-28YE9U | 滅活小腸結腸炎耶爾森氏菌O:9抗原(天然提取物) | x1mg | Inactivated Yersinia enterocolitica O:9 antigen (native extract) |
MT-28YE9Usl | 滅活小腸結腸炎耶爾森氏菌O:9抗原(天然提取物) | x100μg | Inactivated Yersinia enterocolitica O:9 antigen (native extract) |
MT-28SBU | 滅活的鮑氏志賀氏菌抗原(天然提取物) | x1mg | Inactivated Shigella boydii antigen (native extract) |
MT-28SBUsl | 滅活的鮑氏志賀氏菌抗原(天然提取物) | x100μg | Inactivated Shigella boydii antigen (native extract) |
MT-28SDU | 滅活的痢疾志賀氏菌抗原(天然提取物) | x1mg | Inactivated Shigella dysenteriae antigen (native extract) |
MT-28SDUsl | 滅活的痢疾志賀氏菌抗原(天然提取物) | x100μg | Inactivated Shigella dysenteriae antigen (native extract) |
MT-28SFU | 滅活的福氏志賀氏菌抗原(天然提取物) | x1mg | Inactivated Shigella flexneri antigen (native extract) |
MT-28SFUdl | 滅活的福氏志賀氏菌抗原(天然提取物) | x100μg | Inactivated Shigella flexneri antigen (native extract) |
MT-28SSU | 滅活的宋內氏志賀菌抗原(天然提取物) | x1mg | Inactivated Shigella sonnei antigen (native extract) |
MT-28SSUsl | 滅活的宋內氏志賀菌抗原(天然提取物) | x100μg | Inactivated Shigella sonnei antigen (native extract) |
MT-29KOE | 滅活小球隱孢子蟲抗原(天然提取物) | x1mg | Inactivated Cryptosporidium parvum antigen (native extract) |
MT-29KOEsl | 滅活小球隱孢子蟲抗原(天然提取物) | x100μg | Inactivated Cryptosporidium parvum antigen (native extract) |
MT-29HHB | 人血紅蛋白蛋白質(天然提取物) | x1mg | Human Haemoglobin protein (native extract) |
MT-29HHBsl | 人血紅蛋白蛋白質(天然提取物) | x100μg | Human Haemoglobin protein (native extract) |
MT-29HLF | 人乳鐵蛋白蛋白質(天然提取物) | x1mg | Human Lactoferrin protein (native extract) |
MT-29HLF | 人乳鐵蛋白蛋白質(天然提取物) | x100μg | Human Lactoferrin protein (native extract) |
MT-29HTF | 人轉鐵蛋白蛋白質(天然提取物) | x1mg | Human Transferrin protein (native extract) |
MT-29HTFsl | 人轉鐵蛋白蛋白質(天然提取物) | x100μg | Human Transferrin protein (native extract) |
MT-20TSS | 溶血性A鏈球菌抗體 | x1mg | Anti-Strep A Pab |
MT-20TSSsl | 溶血性A鏈球菌抗體 | x100μg | Anti-Strep A Pab |
MT-18RV3 | 呼吸道合胞病毒單抗(克隆RV3) | x1mg | Anti-RSV Mab (clone RV3) |
MT-18RV3sl | 呼吸道合胞病毒單抗(克隆RV3) | x100μg | Anti-RSV Mab (clone RV3) |
MT-18RV4 | 呼吸道合胞病毒單抗(克隆RV4) | x1mg | Anti-RSV Mab (clone RV4) |
MT-18RV4sl | 呼吸道合胞病毒單抗(克隆RV4) | x100μg | Anti-RSV Mab (clone RV4) |
MT-18A14 | 腺病毒單克隆抗體(克隆A14) | x1mg | Anti-Adenovirus Mab (clone A14) |
MT-18A14sl | 腺病毒單克隆抗體(克隆A14) | x100μg | Anti-Adenovirus Mab (clone A14) |
MT-18A15 | 腺病毒單克隆抗體(克隆A15) | x1mg | Anti-Adenovirus Mab (clone A15) |
MT-18A15Rsl | 腺病毒單克隆抗體(克隆A15) | x100μg | Anti-Adenovirus Mab (clone A15) |
MT-18Y77 | 甲型流感病毒單抗(克隆Y77) | x1mg | Anti-Influenza A Mab (clone Y77) |
MT-18Y77sl | 甲型流感病毒單抗(克隆Y77) | x100μg | Anti-Influenza A Mab (clone Y77) |
MT-18YB91 | 乙型流感病毒單抗(克隆YB91) | x1mg | Anti-Influenza B Mab (clone YB91) |
MT-18YB91sl | 乙型流感病毒單抗(克隆YB91) | x100μg | Anti-Influenza B Mab (clone YB91) |
MT-18LN14 | 嗜肺軍團菌單抗(克隆LN14) | x1mg | Anti-Legionella pneumophila Mab (clone LN14) |
MT-18LN14sl | 嗜肺軍團菌單抗(克隆LN14) | x100μg | Anti-Legionella pneumophila Mab (clone LN14) |
MT-18LN29 | 嗜肺軍團菌單抗(克隆LN29) | x1mg | Anti-Legionella pneumophila Mab (clone LN29) |
MT-18LN29sl | 嗜肺軍團菌單抗(克隆LN29) | x100μg | Anti-Legionella pneumophila Mab (clone LN29) |
MT-18SN3 | 肺炎鏈球菌單克隆抗體(克隆SN3) | x1mg | Anti-Streptococcus pneumoniae Mab (clone SN3) |
MT-18SN3s1 | 肺炎鏈球菌單克隆抗體(克隆SN3) | x100μg | Anti-Streptococcus pneumoniae Mab (clone SN3) |
MT-18SN4 | 肺炎鏈球菌單克隆抗體(克隆SN4) | x1mg | Anti-Streptococcus pneumoniae Mab (clone SN4) |
MT-18SN4sl | 肺炎鏈球菌單克隆抗體(克隆SN4) | x100μg | Anti-Streptococcus pneumoniae Mab (clone SN4) |
MT-25RSV | 呼吸道合胞病毒重組融合蛋白 | x1mg | RSV recombinant fusion protein |
MT-25RSVsl | 呼吸道合胞病毒重組融合蛋白 | x100μg | RSV recombinant fusion protein |
MT-25HEXR | 腺病毒六鄰體重組蛋白 | x1mg | Adenovirus HEXON recombinant protein |
MT-25HEXRsl | 腺病毒六鄰體重組蛋白 | x100μg | Adenovirus HEXON recombinant protein |
MT-25FAN | 甲型流感病毒重組核蛋白 | x1mg | Influenza A recombinant nucleoprotein |
MT-25FANsl | 甲型流感病毒重組核蛋白 | x100μg | Influenza A recombinant nucleoprotein |
MT-25FBN | 乙型流感病毒重組核蛋白 | x1mg | Influenza B recombinant nucleoprotein |
MT-25FBNsl | 乙型流感病毒重組核蛋白 | x100μg | Influenza B recombinant nucleoprotein |
MT-28SAGU | 滅活A鏈球菌抗原(天然提取物) | x1mg | Inactivated STREP A antigen (native extract) |
MT-28SAGUsl | 滅活A鏈球菌抗原(天然提取物) | x100μg | Inactivated STREP A antigen (native extract) |
MT-29RVV | 滅活呼吸道合胞病毒抗原(天然提取物) | x1mg | Inactivated RSV antigen (native extract) |
MT-29RVVsl | 滅活呼吸道合胞病毒抗原(天然提取物) | x100μg | Inactivated RSV antigen (native extract) |
MT-28LNU | 滅活的嗜肺軍團菌抗原(天然提取物) | x1mg | Inactivated Legionella pneumophila antigen (native extract) |
MT-28LNUsl | 滅活的嗜肺軍團菌抗原(天然提取物) | x100μg | Inactivated Legionella pneumophila antigen (native extract) |
MT-28SPNU | 滅活的肺炎鏈球菌抗原(天然提取物) | x1mg | Inactivated Streptococcus pneumoniae antigen (native extract) |
MT-28SPNUsl | 滅活的肺炎鏈球菌抗原(天然提取物) | x100μg | Inactivated Streptococcus pneumoniae antigen (native extract) |
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3、zui大簡約法(Maximum parsimony,MP):
zui早源于形態性狀研究,現在已推廣到分子序列的進化分析中。zui大簡約法的理論基礎是奧卡姆(Ockham)哲學原則,這個原則認為:解釋一個過程的理論是所需假設數目zui少的那一個。對所有可能的拓撲結構進行計算,并計算出所需替代數zui小的那個拓撲結構,作為*樹。優點:zui大簡約法對于分析某些特殊的分子數據,如插入、缺失等序列有用。在分析的序列位點上沒有回復突變或平行突變,且被檢驗的序列位點數很大的時候,zui大簡約法能夠推導獲得一個很好的進化樹。缺點:在分析序列上存在較多的回復突變或平行突變,而被檢驗的序列位點數又比較少的時候,zui大簡約法可能會給出一個不合理的或者錯誤的進化樹推導結果。
一般來講,如果模型合適,ML的效果較好。對近緣序列,有人喜歡MP,因為用的假設zui少。MP一般不用在遠緣序列上,這時一般用NJ或ML。對相似度很低的序列,NJ往往出現Long-branch attraction(LBA,長枝吸引現象),有時嚴重干擾進化樹的構建。貝葉斯的方法則太慢。對于各種方法構建分子進化樹的準確性,一篇綜述(Hall BG. Mol Biol Evol 2005, 22(3):792-802)認為貝葉斯的方法,其次是ML,然后是MP。其實如果序列的相似性較高,各種方法都會得到不錯的結果,模型間的差別也不大。
對于NJ和ML,是需要選擇模型的。對于蛋白質序列以及DNA序列,兩者模型的選擇是不同的。對于蛋白質的序列,一般選擇Poisson Correction(泊松修正)這一模型。而對于核酸序列,一般選擇Kimura 2-parameter(Kimura-2參數)模型。如果對各種模型的理解并不深入,不推薦初學者使用其他復雜的模型。
BOOTSTRAP值即自展值,可用來檢驗所計算的進化樹分支可信度。Bootstrap幾乎是構建系統進化樹一個必須的選項。一般Bootstrap的值>70%,則認為構建的進化樹較為可靠。如果Bootstrap的值太低,則有可能進化樹的拓撲結構有錯誤,進化樹是不可靠的。
Bootstrap值是指根據所選的統計計算模型,設定初始值1000次,就是把序列的位點都重排,重排后的序列再用相同的辦法構樹,如此讓模型計算并繪制1000株系統發育樹,這是命令階段產生的。如果原來樹的分枝在重排后構的樹中也出現了,就給這個分枝打上1分,如果沒出現就給0分,這樣給進化樹打分后,每個分枝就都得出分值。系統發育樹中每個節點上的數字則代表在命令階段要求的1000次進化樹分析中,有多少次。重排的序列有很多組合,值越小說明分枝的可信度越低,根據數據的情況選用不同的構樹方法和模型。比如鑒定菌種時一般認為節點數字初始設置1000計算后顯示大于500(有的時候顯示是百分數,要注意)時,這樣的系統發育分析才具有可信度,學術雜志才會接收或者認可。
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3, the maximum parsimony (Maximum parsimony, MP):
Earliest from morphological traits, has now been extended to the evolutionary analysis of molecular sequences. The theoretical basis of Maximal Minimalist Law is the Ockham philosophy, which states that the best theory to explain a process is the one that requires the least number of hypotheses. Calculate all possible topologies and calculate the topology with the smallest number of substitutions as the optimal tree. Advantages: Maximum parsimony is useful for analyzing certain molecular data, such as insertions, deletions, etc. When there are no back mutations or parallel mutations in the analyzed sequence sites, and the number of sequence sites tested is large, the maximum parsimony method can be deduced to obtain a good phylogenetic tree. Disadvantages: When there are many recovery mutations or parallel mutations in the analysis sequence, and the number of sequences to be examined is relatively small, the maximum parsimony method may give an unreasonable or wrong evolutionary tree derivation result.
In general, ML works better if the model is fit. Some people like MPs for the near-miss sequences, because they use the least assumptions. MP is generally not used in the distant sequence, then generally use NJ or ML. For sequences with low similarity, NJ often has Long-branch attraction (LBA), which sometimes seriously interferes with the construction of phylogenetic trees. Bayesian approach is too slow. A review (Hall BG. Mol Biol Evol 2005, 22 (3): 792-802) considers Bayes' approach to be the best, followed by ML and then MP, for the accuracy of the various approaches for constructing molecular phylogenetic trees. In fact, if the similarity of sequences is high, all kinds of methods will get good results, and the difference between the models is not big.
For NJ and ML, it is necessary to choose the model. For protein sequences and DNA sequences, the choice between the two models is different. For protein sequences, the Poisson Correction model is generally chosen. For nucleic acid sequences, the Kimura 2-parameter model is generally chosen. If the understanding of various models is not deep, it is not recommended for beginners to use other complex models.
The BOOTSTRAP value is the self-scaling value that can be used to test the confidence of the calculated branch of the tree. Bootstrap is almost a necessary option for building a phylogenetic tree. General Bootstrap value> 70%, then the construction of the evolutionary tree that more reliable. If the value of Bootstrap is too low, then there is a possibility that the topology of the evolutionary tree is wrong and the evolutionary tree is not reliable.
Bootstrap value is based on the selected statistical model, set the initial value of 1000 times, is to rearrange the sequence of sites, the rearranged sequence and then use the same way to tree, so that the model to calculate and draw 1000 Phylogenetic tree, which is produced during the command phase. If the original tree branches in the rearranged structure of the tree also appeared, give this branch labeled 1 point, if not give 0 points, so after the evolutionary tree scoring, each branch have come Score. The number at each node in the phylogenetic tree represents how many times in the 1000 phylogenetic analyzes required in the command phase. There are many combinations of rearranged sequences, the smaller the value indicates the lower the credibility of the branch, the best according to the situation of the data to choose different tree methods and models. Such as the identification of species is generally believed that the initial calculation of the number of nodes displayed after the calculation of more than 500 (sometimes shown as a percentage, pay attention), such a phylogenetic analysis only has the credibility of academic journals will receive or endorse.