梭菌鑒別瓊脂(DCA)incubation media MH肉湯(MHB) 250(g)
incubation media MH瓊脂(MHA) 250(g)
incubation media 中國藍瓊脂 250(g)
incubation media 克氏雙糖鐵瓊脂(KIA) 250(g)
產(chǎn)品名稱:梭菌鑒別瓊脂(DCA)
英文名稱:Clostridium Differential Agar (DCA)
規(guī)格:250g/瓶
作用:用于干燥食品中亞硫酸鹽還原梭 菌的計數(shù)
型號:FS-PYJ0433
使用范圍:僅供科研使用
庫存:提供現(xiàn)貨(因銷量問題庫存會有變動,具體請與銷售人員確認)
保存:低溫避光保存
梭菌鑒別瓊脂(DCA)的步驟:
(一)準確稱量試劑:根據(jù)不同的菌類和用途,選擇適宜的培養(yǎng)基,培養(yǎng)基所需試劑必須純凈。(二)校正pH值:將稱量好的培養(yǎng)基各種成分放入容器內(nèi),標記劃線,加熱溶解,補充水分,測定酸堿度,常用pH6.8~8.0的精密試紙或酸度計測定。用1N NaOH和1N HCl調(diào)節(jié)pH值到適宜范圍內(nèi)。(三)過濾:將玻璃漏斗置鐵架上,再用紗布夾棉花或用濾紙放在漏斗中,將上述培養(yǎng)基倒入其中過濾至透明。
(四)分裝:將過濾后的培養(yǎng)基分裝于中試管或三角瓶內(nèi)(試管內(nèi)每支裝5mL;三角瓶中裝100~150ml),塞好棉塞用牛皮紙包扎好,準備滅菌。
(五)滅菌:培養(yǎng)基的滅菌,常用高壓蒸汽滅菌法。一般微生物的營養(yǎng)細胞在水中煮沸后即被殺死,但細菌的芽胞有較強的抗熱性,須經(jīng)高壓蒸汽滅菌才能達到*滅菌的目的。根據(jù)蒸汽溫度隨壓力升高而上升的原理,即壓力越大,蒸汽溫度就越高。因此,在同一溫度條件下,采用高壓蒸汽滅菌比干熱滅菌法效果要好。而且在濕熱情況下,菌體吸收水分后,其蛋白質(zhì)易于凝固變性,因為蒸汽的穿透力強,殺菌效果好。
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