本試劑盒應用雙抗體夾心法測定標本中牛防御素β103A(DEFβ103A)測定試劑盒水平。用純化的抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入，再與HRP標記的結合，形成抗體-抗原-酶標抗體復合物，經過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉化成藍色，并在酸的作用下轉化成zui終的黃色。顏色的深淺和樣品中的呈正相關。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品中的濃度。

The E-Z 96? Mag-Bind? Tissue RNA Kit provides a novel technology for total RNA isolation from plant and animal tissues. This kit allows the rapid and reliable isolation of high-quality total cellular RNA from a wide variety of tissues. Samples are first lysed in a specially formulated buffer and then proteins and DNA are precipitated. The Supertanent is combined with magentic beads and after a couple of quick wash steps pure RNA is eluted. Unlike column-based systems, the binding of nucleic acids to magnetic partciles occurs in solution, resulting in increased binding kinetics and binding efficiency. Particles are also completly re-suspended during the wash steps of the purification protocol, which enhances contact with the wash buffer and removal of contaminants and increasing nucleic acid purity. E.Z.N.A.? Mag-Bind? Tissue RNA procedure can be automated with most robotic workstations。

產品說明：方便－處理簡單, 樣品不再需要超低溫貯藏靈活－純化RNA時，只需要將樣品取出即可可靠－該保護液是一種新型的溶液，能快速滲透到細胞或組織內部，高效地抑制內源性RNase酶的活性。

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