利什曼蟲檢測(cè)試劑盒
利什曼蟲leishmanioses的病原體。利什曼蟲屬的許多物種對(duì)人類致病性沒有顯示形態(tài)學(xué)差異。它們可以分化的基礎(chǔ)上生物標(biāo)準(zhǔn),實(shí)驗(yàn)分析了不同臨床圖片和流行病學(xué)的事實(shí)(見標(biāo)簽。)。
利什曼蟲屬Phlebotomus雌蚊的傳播和Lutzomyia(白蛉?),發(fā)生在溫暖地區(qū)的國(guó)家在亞洲、非洲、歐洲地中海國(guó)家和拉丁美洲。無鞭毛體階段的寄生蟲攝取的昆蟲腸道的血被轉(zhuǎn)換成纖細(xì),鞭打promastigote形式,繁殖和遷移回吻。當(dāng)感染白蛉再bloodmeal promastigote形式被接種到一個(gè)新主機(jī)(人類或其它脊椎動(dòng)物)。
在宿主promastigotes迅速轉(zhuǎn)變成無鞭毛體階段(12 - 14小時(shí)內(nèi)),然后釋放過程類似胞外分泌和可以感染新的細(xì)胞。在皮膚和黏膜與皮膚的leishmanioses寄生蟲一般仍局限于皮膚或皮膚和粘膜。皮膚leishmanioses病變可能持續(xù)很長(zhǎng)一段時(shí)間,但往往自發(fā)愈合,而更傾向于破壞性變化在黏膜與皮膚的leishmanioses感染。相比之下在內(nèi)臟leishmanioses有機(jī)體可以入侵整個(gè)單核吞噬系統(tǒng)各器官(脾、肝、淋巴結(jié)、節(jié)點(diǎn)、骨髓、血液?jiǎn)魏思?xì)胞等),導(dǎo)致感染,沒有治療通常是致命的。
物種 | 疾病 | 癥狀 | 感染的機(jī)制 |
嬰兒利什曼蟲 杜氏利什曼蟲 | 內(nèi)臟leishmanioses(黑熱病) | 發(fā)熱、脾腫大、hypergammaglobulinemia進(jìn)行性貧血、白細(xì)胞減少等。 | 傳輸?shù)拇莆脤貾hlebotomus(舊世界)和Lutzomyia(新世界),稱為?白蛉?。 |
利什曼蟲主要 | 皮膚leishmanioses(東方痛) | 丘疹,船只和節(jié)點(diǎn) | |
利什曼蟲取代巴西橡膠樹 | 黏膜與皮膚的leishmanioses | 皮膚的變化類似于東方痛。某些形式往往會(huì)擴(kuò)散到粘膜,引起嚴(yán)重的組織破壞。 | |
利什曼蟲墨西哥 | 皮膚leishmanioses | 慢性病變類似于麻風(fēng)病 |
感染的診斷則需要通過:
顯微鏡:
測(cè)定有色Giemsa-stained涂片的病原體
血清學(xué):
確定特定的基于ELISA-technique抗體
NovaLisa嗎?嬰兒利什曼蟲免疫球蛋白ELISA:
NovaLisa嗎?嬰兒利什曼蟲免疫球蛋白ELISA用于定性測(cè)定IgG-class嬰兒利什曼蟲抗體在人類血清或血漿(檸檬酸)。
抗原:
純化利什曼蟲抗原
具體的性能特征:
| Intraassay | Interasay | 靈敏度 | 特異性 | ||||
| n | 的意思是 | CV % | n | 的意思是 | CV % |
|
|
免疫球蛋白 | 7 | 1.609 | 7.4 | 4 | 0.735 | 5.9 | 91年 | 85年 |
訂單信息:
ELISA | 的數(shù)量決定 | 產(chǎn)品編號(hào) |
嬰兒利什曼蟲免疫球蛋白 | 96年 | LEIG0310 |
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Leishmania infantum
Leishmania is the causative agent of leishmanioses. The many species of the genus Leishmania pathogenic to humans do not show morphological differences. They can be differentiated on the basis of biological criteria, laboratory analyzes the different clinical pictures and epidemiological facts (see tab.).
Leishmania are transmitted by female mosquitoes of the genera Phlebotomus and Lutzomyia (?sandflies?), that occur in warmer regions of countries in Asia, Africa, Europe (Mediterranean countries) and Latin America. The amastigote stages of the parasite ingested by the insect with a blood meal are transformed in its intestine into slender, flagellate promastigote forms, which multiply and migrate back into the proboscis. When infected sandflies take another bloodmeal the promastigote forms are inoculated into a new host (humans or other vertebrates).
In the host the promastigotes quickly (within 12-14 hours) transform into amastigote stages, which then released in a process resembling exocytosis and can infect new cells. In cutaneous and mucocutaneous leishmanioses the parasites generally remain restricted to the skin or skin and mucosa. Cutaneous leishmanioses lesions may persist for long periods, but tend to heal spontaneously, whereas a greater tendency to destructive changes is seen in mucocutaneous leishmanioses infections. By contrast in visceral leishmanioses the organism can invade the entire mononuclear phagocytic system in various organs (spleen, liver, lymph, nodes, bone marrow, blood monocytes etc.), causing infections that are normally lethal without treatment.
Species | Disease | Symptoms | Mechanism of Infection |
Leishmania infantum Leishmania donovani | Visceral leishmanioses (Kala-azar) | Fever, splenomegaly, hypergammaglobulinemia, progressive anemia, leucopenia etc. | Transmission by female mosquitoes of the genera Phlebotomus (Old World) and Lutzomyia (New World), known as ?sandflies?. |
Leishmania major | Cutaneous leishmanioses (oriental sore) | Papules, vessels and nodes | |
Leishmania brasiliensis | Mucocutaneous leishmanioses | Skin changes similar to oriental sore. Some forms tend to spread to mucosa and cause severe tissue destruction. | |
Leishmania mexicana | Cutaneous leishmanioses | Chronic lesions similar to leprosy |
Infections may be diagnosed by:
Microscopy:
determination of the pathogen in coloured Giemsa-stained smears
Serology:
determination of specific antibodies based on the ELISA-technique
NovaLisa? Leishmania infantum IgG ELISA:
The NovaLisa? Leishmania infantum IgG ELISA is intended for the qualitative determination of IgG-class antibodies against Leishmania infantum in human serum or plasma (citrate).
Antigens:
Purified Leishmania antigens
Specific performance characteristics:
| Intraassay | Interasay | Sensitivity | Specificity | ||||
| n | Mean | CV% | n | Mean | CV% |
|
|
IgG | 7 | 1.609 | 7.4 | 4 | 0.735 | 5.9 | 91 | 85 |
Order information:
ELISA | Number of Determinations | Product Number |
Leishmania infantum IgG | 96 | LEIG0310 |
