ELISA是以免疫學反應為基礎，將抗原、抗體體的特異性反應與酶對底物的高效催化作用相結合起來的一種敏感性很高的試驗技術。

人RAS癌基因家族成員RAB1A(RAB1A)測定盒是北京方程生物公司的優勢產品，2017年買人RAS癌基因家族成員RAB1A(RAB1A)測定盒送好禮*活動火熱進行中......

人RAS癌基因家族成員RAB1A(RAB1A)測定盒

Human RAB1A, Member RAS Oncogene Family (RAB1A) ELISA

Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.

人RAS癌基因家族成員RAB1A(RAB1A)測定盒操作步驟：1. 編號：將樣品對應微孔按序編號，每板應設陰性對照 2 孔、陽性對照 2 孔、空白對照 1孔（空白對照孔不加樣品及酶標試劑，其余各步操作相同）2. 加樣：分別在陰、陽性對照孔中加入陰性對照、陽性對照 50μl。然后在待測樣品孔先加樣品稀釋液 40μl，然后再加待測樣品 10μl。加樣將樣品加于酶標板孔底部，盡量不觸及孔壁，輕輕晃動混勻，3. 溫育：用封板膜封板后置 37℃溫育 30 分鐘。4. 配液：將 30（48T 的 20 倍）倍濃縮洗滌液加蒸餾水至 600ml 后備用5. 洗滌：小心揭掉封板膜，棄去液體，甩干，每孔加滿洗滌液，靜置 30 秒后棄去，如此重復 5 次，拍干。6. 加酶：每孔加入酶標試劑 50μl，空白孔除外。7. 溫育：操作同 3。8. 洗滌：操作同 5。9. 顯色：每孔先加入顯色劑 A 50μl，再加入顯色劑 B 50μl，輕輕震蕩混勻，37℃避光顯色15 分鐘10. 終止：每孔加終止液 50μl，終止反應（此時藍色立轉黃色） 。11. 測定：以空白空調零，450nm 波長依序測量各孔的吸光度（OD 值） 。 測定應在加終止液后 15 分鐘以內進行。

人RAS癌基因家族成員RAB1A(RAB1A)測定盒

人凋亡蛋白酶激活因子1(APAF1)測定盒Human Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA

豬尿酸鹽轉運蛋白1(URAT1)檢測盒Pig Urate Transporter 1 (URAT1) ELISA

豬巨噬細胞炎性蛋白1α(MIP1α)檢測盒Pig Macrophage Inflammatory Protein 1 Alpha (MIP1a) ELISA

人干細胞因子(SCF)測定盒Human Stem Cell Factor (SCF) ELISA

豬細胞因子受體樣因子1(CRLF1)檢測盒Pig Cytokine Receptor Like Factor 1 (CRLF1) ELISA

豬蛋白酶激活亞基3(PSME3)檢測盒Pig Proteasome Activator Subunit 3 (PSME3) ELISA

人生長激素2(GH2)測定盒Human Growth Hormone 2 (GH2) ELISA

豬脂多糖結合蛋白(LBP)檢測盒Pig Lipopolysaccharide Binding Protein (LBP) ELISA

人血管緊張素Ⅱ(ANGⅡ)測定盒Human Angiotensin II (AngII) ELISA

人組織金屬蛋白酶抑制因子4(TIMP4)測定盒Human Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) ELISA

人干擾素調節因子2結合蛋白2(IRF2BP2)測定盒Human Interferon Regulatory Factor 2 Binding Protein 2(IRF2BP2) ELISA

人生長因子受體結合蛋白14(Grb14)測定盒Human Growth Factor Receptor Bound Protein 14 (Grb14) ELISA