ELISA是以免疫學反應為基礎,將抗原、抗體體的特異性反應與酶對底物的高效催化作用相結合起來的一種敏感性很高的試驗技術。
抗丙型肝炎病毒抗體(anti-HCV)是北京方程生物公司的優勢產品,2017年買抗丙型肝炎病毒抗體(anti-HCV)送好禮*活動火熱進行中......
抗丙型肝炎病毒抗體(anti-HCV)
甘露糖受體
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抗丙型肝炎病毒抗體(anti-HCV)Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.
抗丙型肝炎病毒抗體(anti-HCV)
血紅素氧合酶1(HO-1)
促卵泡素(FSH)
血管緊張素Ⅰ轉化酶(ACEⅠ)
丙酮酸脫氫酶E1(PDH E1)
細胞間粘附分子3(ICAM-3/CD50)
T細胞特異性鳥苷三磷酸酶(Tgtp)
牛小腸堿性磷酸酶(CIAP)
多聚血清蛋白(PHSA)
抗眼肌抗體(EMAb)
組織型纖溶酶原激活劑(t-PA)
抗糖蛋白抗體(GP)
轉化生長因子β1(TGF-β1)