CellROX™ Green Reagent, for oxidative stress detection
氧化應激檢測用探針（綠色）
訂購信息：
貨號 | 產品名稱 | Ex/Em(nm) | 規格 | 價格（元） | 是否小包裝供貨 |
C10423 | CellROX Orange Reagent 橙色 | 545/565 | 5 x 50 µL | 7214 | 否 |
C10444 | CellROX Green Reagent 綠色 | 485/520 | 5 x 50 µL | 7175 | 是（50 µl） |
C10422 | CellROX Deep Red Reagent 深紅色 | 640/665 | 5 x 50 µL | 7156 | 否 |
以上所有產品均為Life（Invitrogen）原裝正品，原始包裝為10x50 µg（10支50µg單個包裝構成）。我司對C10444進行拆開銷售，特惠價為1845元/支，單支50µl，現貨銷售，歡迎訂購。
背景介紹：
氧化應激（Oxidative stress）的產生，起因于反應氧物質（ROS）生成與細胞清除ROS之間的不平衡。ROS在幾種疾病的發展中發揮重要作用，包括炎癥、動脈粥樣硬化、衰老和衰老相關的退行性疾病。
基本介紹：
CellROX® Green Reagent是一種檢測活細胞氧化應激的新型熒光探針。此細胞膜滲透性的染料在還原態時呈弱熒光，一旦被活性氧物質ROS氧化并結合到DNA后發明亮的綠色熒光，具有~485/520nm 的zui大激發/發射波長。此染料能夠被甲醛固定且能承受去污劑處理，因此特別適合與其他兼容性染料和抗體進行多重標記檢測。
CellROX® Green Reagent與各種檢測平臺兼容，比如傳統熒光顯微鏡、高內涵篩選儀器（HCS）、流式細胞儀、熒光酶標儀或高通量篩選（HTS）。還與各種臺式儀器兼容，比如FLoid™，Tali™，和Attune™儀器。
產品參數
外觀：淺黃色溶液（pale yellow solution）
溶劑：DMSO
激發/發射光譜范圍：可見光/可見光
亞細胞定位：線粒體、細胞核
保存方法：≤-20°C避光干燥
染色示例
Fig1. Nefazodone Induced Oxidative Stress Measured with CellROX™ Green Reagent in HepG2 Cells Nefazodone induced oxidative stress in hepatocarcinoma (HepG2) cells measured with CellROX™ Green Reagent. HepG2 cells were plated on glass bottom 35 mm MatTek dishes. The cells were treated with or without 50 µM nefazodone for 24 hr at 37° C. The cells were then stained with 5 µM CellROX™ Orange Reagent and Hoechst 33342 by adding the probes to complete media and incubating at 37° C for 30 min. The cells were washed with PBS and imaged on a Zeiss Axiovert inverted microscope using a 40x objective. Nefazodone treatment leads to increased oxidative stress.
Fig2. MnTBAP Inhibition of Menadione Induced Oxidative Stress Measured with CellROX™ Green Reagent in BPAE Cells
MnTBAP inhibition of menadione induced oxidative stress in bovine pulmonary endothelial (BPAE) cells measured with CellROX™ Green Reagent. BPAE cells were plated on glass bottom 35 mm MatTek dishes. The cells were treated with or without 100 µM menadione for 1 hr at 37° C. 100 µM MnTBAP was added to the dish with or without menadione treatment. The cells were then stained with 5 µM CellROX™ Green Reagent and Hoechst 33342 by adding the probes to complete media and incubating at 37° C for 30 min. The cells were washed with PBS and imaged on a Zeiss Axiovert inverted microscope using a 40x objective. MnTABP co-incubation with menadione reduced the oxidative stress in the cells.
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上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作，主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發生產等領域。 本公司秉承“以人為本，以誠為信、合同守信”的經營理念。堅持"品質保障"的原則為廣大客戶提供優質產品。
CellROX™ Green Reagent 氧化應激檢測探針
CellROX™ Green Reagent 氧化應激檢測探針
