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技術文章

26S Proteasome Fraction抗體說明書

閱讀:353發布時間:2013-4-9

26S Proteasome Fraction抗體說明書

FROM: GeneTex

Catalog Number: GTX80879

Background: Mammalian 26S proteasome purified through a specific series of specialized chromatographicsteps yields a partially purified fraction containing 26S proteasome and no conjugation enzymes(E1, E2s, and E3s). This stable fraction is preferred for the degradation of pre-formed ubiquitinproteinconjugates by the 26S proteasome since highly purified 26S is costly and more labile.26S concentration is estimated based on analytical SEC at 1500 kDa and quantitation of the 20Score particle activity using a fluorogenic peptide substrate.

 

Item   : 26S Proteasome Fraction

 

Quantity size:0.2ml

 

Format: Estimated 26S concentration = X nM. Concentration will vary with Lot#.Buffer: 50 mM Hepes pH 7.6, 1 mM MgATP, 1 mM DTT,150 mM NaCl, 10 % glycerol.

 

Species:  rabbit

 

Host   :  rabbit reticulocytes

 

Source/Expression System:  rabbit

 

References

 

Driscoll J. and Goldberg A.L. (1990) J. Biol. Chem. 265:4789-4792 Ganoth D., et al. (1988) J. Biol. Chem. 263:12412-12419 Voges D., et al. (1999) Ann. Rev. Biochem. 68:1015-1068 Waxman L., et al. (1987) J. Biol. Chem. 262:2451-2457

 

Specifications/Features

 

For best results, aliquot and freeze after first set of experiments to avoidmultiple freeze/thaw cycles. Typical concentrations for use are 5-50 nMdepending on conditions.

 

26S Proteasome Fraction抗體說明書

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