Rabbit Anti- TRPV5抗體說明書 antibody
FROM:Alpha Diagnoestic International
Catalog Number : CAT21-A
Quantity size : 0.1ml (dilute with pH7.4 0.01 M PBS or antibody diluent )
Background: Transient receptor potential (TRP) proteins are cation-sensitive channels that modulate a myriad of cellular functions, including temperature sensation and vasoregulation Transcribed from a gene adjacent to VR-1, the thermal-sensitive, capsaicin-insensitive TRPV3 is expressed at warm temperatures; expression increases in response to noxious temperatures. Human TRPV3 is expressed in skin, tongue, dorsal root ganglion, trigeminal ganglion, spinal cord and brain. In addition, TRPV3 is co-expressed in dosal root ganglion neurons with VR-1. TRPV3 associates with VR-1 and may modulate VR-1 activity. The 729 amino acid TRPV5 (ECAC1) protein comprises six transmembrane domains, multiple potential phosphorylation sites, an N-linked glycosylation site and three ankyrin repeat regions. It is abundantly expressed in kidney, jejunum and pancreas, and at lower levels in testis, prostate, placenta, brain, colon and rectum. TRPV5 controls the rate-limiting step of vitamin D3-regulated Ca2+ reabsorption in kidney and intestine; the 5’-flanking region of TRPV5 contains four putative vitamin D3-responsive elements.
Function:TRPV5抗體說明書
Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating.
pecificity :
– Anti-TRPV5 is a rabbit polyclonal antibody unconjugated
– specific for TRPV5 of human,rat,mouse
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein A affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 83 kDa
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– ELISA 1:500-1000
– Optimal working dilutions must be determined by the end user.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The
lyophilized antibody is stable at room temperature for at least one month and for
greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M
PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃TRPV5抗體說明書
Important Note: This product as supplied is intended for research use only, not for
use in human, therapeutic or diagnostic applications.
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