Anti-CD4 /FITC標(biāo)記抗體
From:ABCAM
Catalog Number : RS- 0647R-FITC
Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent ) Background: The CD4 antigen is highly expressed on human T helper cells and thymocytes, and at lower levels on monocytes and dendritic cells. This integral membrane glycoprotein of approximay 58 kDa contains four external domains (D1 to D4) that show homology to members of the immunoglobulin (Ig) superfamily. It is responsible for the recognition of the MHC class II antigen. The CD4 antibody recognizes most thymocytes and about 65% of peripheral blood T cells.
Function : Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Subunit : Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.
Subcellular Location : Cell membrane; Single-pass type I membrane protein.
Post-translational modifications : Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Similarity : Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
.Specificity : Anti- CD4/FITC is a rabbit polyclonal antibody
specific for CD4/FITCof Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig, ,MACFA
- use for western blotting,elisa,immunoprecipitation and immunohistochemistry
- Protein G affinity chromatography purification, purity :>95%
- Isotype: IgG
- mol wt: 48kDa
- Application :
- IF=1:50-200
- KLH conjugated synthetic peptide derived from human CD4
- not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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