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技術(shù)文章

H2Ax抗體

閱讀:403發(fā)布時(shí)間:2013-10-3

Anti-H2Ax抗體

From:ABCAM

Catalog Number :RS-2560R

Quantity size :   0.1ml  (dilute with pH 7.4    0.01 M PBS or antibody diluent ) Background: Annexin V (ANX5) is a member of a family of calcium-dependent phospholipid binding proteins. This family of annexins also includes giardin that has been shown to function as an annexin; pfam00191

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximay 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq].

Specificity : Anti-H2Ax a rabbit polyclonal antibody              

specific for H2Ax of   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,

  • use for western blotting,elisa,immunoprecipitation and immunohistochemistry
  • Protein G affinity chromatography purification, purity :>95%
  • Isotype:  IgG
  • mol wt:  16kDa

Application :  

  • WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  • not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user..

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

 

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.

 

 


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