Anti-Osteocalcin/FITC說明書
Catalog Number :Rs-4917R-FITC
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ） Background:
Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite. Function : Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.Subcellular Location : Secreted.Post-translational modifications : Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
Similarity : Belongs to the osteocalcin/matrix Gla protein family.
Contains 1 Gla （gamma-carboxy-glutamate） domain.
Specificity : Anti-Osteocalcin/FITC is a mouse Monoclonall antibody
specific for Osteocalcin/FITCof Human, Rb
- use for western blotting,elisa,immunoprecipitation and immunohistochemistry
- Protein G affinity chromatography purification, purity :>95%
- Isotype: IgG
- mol wt: 11kDa
Application :
- Flow-cyt=1:50-200 IF=1:50-200
- Emission spectrum:490-510nm
- not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
