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尼古丁快速檢測試劑卡
廣州健侖生物科技?有限公司
本司長期供應尼古丁(可替寧)檢測試劑盒,其主要品牌包括美國NovaBios、廣州健侖、廣州創侖等進口產品,國產產品,試劑盒的實驗方法是膠體金方法。
我司還提供其它進口或國產試劑盒:登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。
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【檢驗原理】本品采用競爭抑制法和膠體金免疫層析法原理定性檢測尿液中的尼古丁,以金標尼古丁單克隆抗體作為指示標記物,在硝酸纖維素膜上的檢測線處和控制線處分別包被尼古丁-BSA結合物和羊抗鼠IgG多克隆抗體。檢測時,尿樣在毛細效應下層析。如尿樣中的尼古丁濃度低于200ng/mL時,金標抗體不能全部與尼古丁結合,未結合的金標抗體在層析過程中與固定在檢測線處的尼古丁-BSA結合物結合,從而在檢測區(T)出現一條紫紅色條帶;如尿樣中尼古丁濃度高于200ng/mL時,金標抗體全部與尼古丁結合,從而在檢測區(T)因為競爭反應不會與尼古丁-BSA結合物結合而不出現紫紅色條帶。無論尿樣中是否存在尼古丁,控制區(C)都會出現一條紫紅色條帶。控制區(C)所呈現的紫紅色條帶是判斷是否有足夠的尿樣,層析過程是否正常的標準,同時也作為試劑的內控標準。
【主要組成成份】
檢測需要但未提供的材料:
【儲存條件及有效期】
儲存條件:原包裝應儲存于4~30℃避光干燥處,切忌冷凍。
有效期:24個月。
試劑盒應在鋁箔袋拆封后1小時內盡快使用;建議在周圍溫度高于30℃或高濕度條件下,盡可能做到即開即用。
【樣本要求】
【檢驗方法】
在進行檢測前必須先完整閱讀使用說明書,使用前將本品和尿樣恢復至室溫(20℃~30℃)。
尼古丁快速檢測試劑卡
2014年,Romesberg團隊在這一領域獲得了突破性的進展,他們在實現了dNaM-d5SICS的人造堿基對的高效高保真體外PCR復制和轉錄之后,隨即啟動了將這一人工堿基對應用于大腸桿菌體內的研究計劃。盡管在體外表現優異,但要將其成功的應用到生物體內顯然還必須面對更多的挑戰。首先,dNaM和d5SICS*是由人工創造出來的非天然堿基,因而Romesberg團隊面臨的*個難題是如何保證細胞內有足夠的dNaMTP以及d5SICSTP單體以用于這種人工DNA的復制。為了解決這一問題,Romesberg FE研究組嘗試了一系列不同來源的核苷三磷酸轉運蛋白,zui終發現一種海藻葉綠體的核苷三磷酸轉運蛋白PtNTT2能夠高效地將添加在培養基中的dNaMTP和d5SICSTP單體運輸到細胞質中。隨后,通過向培養基中添加磷酸鉀,該團隊又有效緩解了這兩種人工合成核苷三磷酸在培養基以及細胞內容易降解的問題。
此后,該團隊構建了一個含有一對人工堿基對的質粒pINF,并且系統地研究了這個質粒在大腸桿菌中的復制情況研究發現,在開啟轉運蛋白PtNTT2表達以及培養基中添加相應的外源人工核苷三磷酸的情況下,這一半合成質粒能夠以適當的速度和準確度進行復制,并且幾乎不阻礙大腸桿菌細胞生長,也沒有明顯表現出其他失去非天然堿基對的跡象。研究結果顯示,這一半合成質粒中的非天然堿基對的復制保真性超過了99.4%,這一保真性與某些病毒的DNA聚合酶相當。新堿基至少復制了24輪,并維持了近一周時間。當沒有這一轉運蛋白表達或是沒有新堿基提供時,非天然堿基d5SICS和dNaM將會逐漸被天然堿基A、T、C、G所替換,并zui終從基因組中*消失。而這種堿基替換已被證明源于DNA復制中的堿基錯配而不是細胞內的DNA修復系統。
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二維碼掃一掃
【公司名稱】 廣州健侖生物科技有限公司
【】 楊永漢
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【騰訊 】
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-3室
【企業文化宣傳】
In 2014, the Romesberg team made a breakthrough in this area, immediay following the introduction of efficient, high-fidelity in vitro PCR replication and transcription of dNaM-d5SICS artificial base pairs Research program in E. coli. Despite its excellent in vitro performance, its successful application to the organism obviously has to face more challenges. First, dNaM and d5SICS are compley engineered non-native bases. The first challenge the Romesberg team faced was how to ensure that enough dNaMTP and d5SICSTP monomers were available in the cell for the replication of this artificial DNA. In order to solve this problem, Romesberg FE team tried a series of nucleoside triphosphate transporters from different sources, finally found that a seaweed chloroplast nucleoside triphosphate transporter PtNTT2 can efficiently add dNaMTP and d5SICSTP monomer is transported into the cytoplasm. Subsequently, by adding potassium phosphate to the medium, the team effectively ameliorated the problems of the two synthetic nucleoside triphosphates being easily degraded in the medium and cells.
Since then, the team has constructed a plasmid pINF containing a pair of artificial base pairs and systematically studied the replication of this plasmid in E. coli. The study found that opening the transporter PtNTT2 expression and culture medium to add the corresponding exogenous In the case of artificial nucleoside triphosphates, this semi-synthetic plasmid replicates with reasonable speed and accuracy with little or no inhibition of E. coli cell growth and no other signs of loss of non-native base pairs. The results show that the fidelity of the unnatural base pairs in this semi-synthetic plasmid exceeds 99.4%, a fidelity equivalent to that of some viral DNA polymerases. The new bases were replicated for at least 24 rounds for nearly a week. In the absence of this transporter expression or absence of new bases, the non-native bases d5SICS and dNaM will gradually be replaced by the native bases A, T, C, G and eventually disappear compley from the genome. Such base substitutions have been shown to result from base mismatches in DNA replication rather than intracellular DNA repair systems.
On May 7, 2014, a research team led by Romesberg gave an online priority online paper on Nature, announcing the birth of a semi-synthetic hexa-nucleic acid molecule containing a pair of artificial base pairs (dNaM-d5SICS). During the same period, Nature distributed comments on the work, and Science, published on May 9, commented on the work. This work can be said to be one of the most important "milestones" in the field of artificial bases so far, marking a formal entry into the body of the research on artificial base pairs.
Since Clostridium perfringens is widespread in nature and its toxins may also be present in the gut of immunized or naturally resistant animals. Therefore, clinical examination of Clostridium perfringens toxins from the gut is still not confirmed to be Clostridium perfringens. Clostridium perfringens Clostridium perfringens According to the epidemiological characteristics of the disease, clinical and pathological features can make a preliminary diagnosis, the diagnosis will have to carry out laboratory tests.
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