胰多肽(PP)檢測(cè)試劑盒
適用生物 Homo sapiens (Human，人)
胰多肽(PP)檢測(cè)試劑盒檢測(cè)范圍 37.04-3000pg/mL 靈敏度 13.8pg/mL
樣本類型 Serum, plasma and other biological fluids.
實(shí)驗(yàn)時(shí)長(zhǎng) 2.5h 實(shí)驗(yàn)方法 競(jìng)爭(zhēng)抑制法 胰多肽(PP)檢測(cè)試劑盒規(guī)格 96T
ELISA Kit for Pancreatic Polypeptide (PP)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. hzEB265Hu
Sample type Serum, plasma and other biological fluids.
Format 96-well strip plate
Assay length 2.5 hours
Detection range 37.04-3000pg/mL The standard curve concentrations used for the ELISA’s were 3000pg/mL, 1000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL
Sensitivity The minimum detectable dose of this kit is typically less than 13.8pg/mL.
胰多肽(PP)檢測(cè)試劑盒Specificity
This assay has high sensitivity and excellent specificity for detection of Pancreatic Polypeptide (PP).
No significant cross-reactivity or interference between Pancreatic Polypeptide (PP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Pancreatic Polypeptide (PP) and the recovery rates were calculated by comparing the measured value to the expected amount of Pancreatic Polypeptide (PP) in samples.
Matrix Recovery range (%) Average(%)
serum(n=5) 88-95 91
EDTA plasma(n=5) 81-104 93
heparin plasma(n=5) 90-103 98
胰多肽(PP)檢測(cè)試劑盒Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pancreatic Polypeptide (PP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pancreatic Polypeptide (PP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pancreatic Polypeptide (PP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-104% 94-102% 89-97% 82-99%
EDTA plasma(n=5) 90-98% 97-104% 91-101% 80-102%
heparin plasma(n=5) 92-101% 92-101% 86-104% 84-91%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
胰多肽(PP)檢測(cè)試劑盒Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120μL Assay Diluent B 1×12mL
Reagent Diluent 1×300μL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50μL standard or sample to each well.
And then add 50μL prepared Detection Reagent A immediay.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50μL Stop Solution. Read at 450 nm immediay.
胰多肽(PP)檢測(cè)試劑盒Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Pancreatic Polypeptide (PP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Pancreatic Polypeptide (PP) and unlabeled Pancreatic Polypeptide (PP) (Standards or samples) with the pre-coated antibody specific to Pancreatic Polypeptide (PP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Pancreatic Polypeptide (PP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Pancreatic Polypeptide (PP) in the sample.
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