mES*培養液配方(600 ml): DMEM (Invitrogen 12430) 497 ml ES級FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl(終濃度為1000U/ml) β-Mer (Invitrogen 21985) 1.5 ml 氣相:空氣,95%;二氧化碳,5%。溫度:37攝氏度。
供應限制
A。僅供研究之用。
REFERENCE
Matise M, et al., Production of targeted embryonic stem cell clones. In: Matise M, et al., Gene Targeting: A Practical Approach, Oxford, Oxford University Press, 101-132, 1999 Xian HQ, et al. A subset of ES-cell-derived neural cells marked by gene targeting. Stem Cells 21: 41-49, 2003. PubMed: 12529550