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北京方程嘉鴻科技有限公司
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北京方程嘉鴻科技有限公司
閱讀：90發(fā)布時(shí)間：2017-4-11
ELISA是以免疫學(xué)反應(yīng)為基礎(chǔ)，將抗原、抗體體的特異性反應(yīng)與酶對(duì)底物的催化作用相結(jié)合起來(lái)的一種敏感性很高的試驗(yàn)技術(shù)。
17-酮類固醇(17-KS)檢測(cè)說(shuō)明書是北京方程生物公司的優(yōu)勢(shì)產(chǎn)品，2017年買17-酮類固醇(17-KS)送好禮*活動(dòng)火熱進(jìn)行中......
17-酮類固醇(17-KS)檢測(cè)說(shuō)明書
17羥孕酮
Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.
17-酮類固醇(17-KS)elisa試劑盒回收率是反應(yīng)待測(cè)物在樣品分析過(guò)程中的損失的程度，損失越少，回收率越高，如果作標(biāo)液1PPM，就是1毫克/升，而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升，就是說(shuō)你的回收率是99%，這個(gè)與真實(shí)成分有密切的關(guān)系，說(shuō)明方法的準(zhǔn)確度。比如水中總無(wú)機(jī)氯含量測(cè)定，樣品水中含有無(wú)機(jī)氯20mg/L，取100mL被測(cè)水樣品，加入0.1mL濃度為10mg/mL的含無(wú)機(jī)氯標(biāo)準(zhǔn)樣品，測(cè)定時(shí)忽略體積變化，如果測(cè)定出樣品中無(wú)機(jī)氯為2.98mg/L，則認(rèn)為回收率為99%。
17-酮類固醇(17-KS)檢測(cè)說(shuō)明書
鉤端螺旋體IgG(Lep IgG)
游離血紅蛋白(f-Hb)
單純皰疹病毒抗原2(HSV-Ag2)
糖鏈抗原19-9(CA19-9)
基質(zhì)金屬蛋白酶抑制因子3(TIMP-3)
硫氧化還原蛋白(Trx)
6-羥多巴胺(6-OHDA)
甲狀腺過(guò)氧化物酶(TPO)
*受體(TR)
癌胚鐵蛋白(CEF)
鳥苷酸交換因子(GEF)
β位淀粉樣前體蛋白裂解酶2(BACE2)
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