通信電纜 網(wǎng)絡(luò)設(shè)備 無線通信 云計(jì)算|大數(shù)據(jù) 顯示設(shè)備 存儲(chǔ)設(shè)備 網(wǎng)絡(luò)輔助設(shè)備 信號傳輸處理 多媒體設(shè)備 廣播系統(tǒng) 智慧城市管理系統(tǒng) 其它智慧基建產(chǎn)品
北京方程嘉鴻科技有限公司
閱讀:276發(fā)布時(shí)間:2017-7-21
人*原C(PGC)測定盒
中文名稱:人*原C(PGC)測定盒
把時(shí)間留給思考,把實(shí)驗(yàn)交給方程生物,專業(yè)的人做專業(yè)的事情,更!
本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中人*原C(PGC)測定盒水平。用純化的抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入,再與HRP標(biāo)記的結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(OD值),通過標(biāo)準(zhǔn)曲線計(jì)算樣品中的濃度。
加樣在ELISA中一般有3次加樣步聚,即加標(biāo)本,加酶結(jié)合物,加底物。加樣時(shí)應(yīng)將所加物加在LEISA板孔的底部,避免加在孔壁上部,并注意不可濺出,不可產(chǎn)生氣泡。加標(biāo)本一般用微量加樣器,按規(guī)定的量加入板孔中。每次加標(biāo)本應(yīng)更換吸嘴,以免發(fā)生交叉污染,也可用一次性的定量塑料管加樣。有此測定(如間接法ELISA)需用稀釋的血清,可在試管中按規(guī)定的稀釋度稀釋后再加樣。也可在板孔中加入稀釋液,再在其中加入血清標(biāo)本,然后在微型震蕩器上震蕩1分鐘以保證混和。加酶結(jié)合物應(yīng)用液和底物應(yīng)用液時(shí)可用定量多道加液器,使加液過程迅速完成。
The E-Z 96? Mag-Bind? Tissue RNA Kit provides a novel technology for total RNA isolation from plant and animal tissues. This kit allows the rapid and reliable isolation of high-quality total cellular RNA from a wide variety of tissues. Samples are first lysed in a specially formulated buffer and then proteins and DNA are precipitated. The Supertanent is combined with magentic beads and after a couple of quick wash steps pure RNA is eluted. Unlike column-based systems, the binding of nucleic acids to magnetic partciles occurs in solution, resulting in increased binding kinetics and binding efficiency. Particles are also completly re-suspended during the wash steps of the purification protocol, which enhances contact with the wash buffer and removal of contaminants and increasing nucleic acid purity. E.Z.N.A.? Mag-Bind? Tissue RNA procedure can be automated with most robotic workstations。
相關(guān)指標(biāo):
人5羥色胺(5-HT)測定盒北京代測
人血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)測定盒
人*原C(PGC)測定盒
人神經(jīng)調(diào)節(jié)蛋白1(NRG-1)測定盒
人鐵蛋白(FE)測定盒北京代測
人17羥孕酮(17-OHP)測定盒北京代測
人熱休克蛋白27(HSP-27)測定盒
人八聚體轉(zhuǎn)錄因子(OTF2A)測定盒
人*酯轉(zhuǎn)運(yùn)蛋白(CETP)測定盒
人前列腺素D合成酶(PGDS)測定盒
人血血小板衍生生長因子可溶性受體α(PDGFsR-α)測定盒
人高敏甲狀腺素(u-T4)測定盒ELISA
商鋪:http://www.cmr6829.com/st173529/
主營產(chǎn)品:胎牛血清,ELISA試劑盒,gibco胎牛血清10099141,胎牛血清16000-044,葡聚糖凝膠a-25,葡聚糖凝膠G-25,小鼠ELISA試劑盒,大鼠ELISA試劑盒,白介素6試劑盒
智慧城市網(wǎng) 設(shè)計(jì)制作,未經(jīng)允許翻錄必究 .? ? ?
請輸入賬號
請輸入密碼
請輸驗(yàn)證碼
請輸入你感興趣的產(chǎn)品
請簡單描述您的需求
請選擇省份