人70kDa熱休克蛋白5(HSPA5)測(cè)定試劑盒
中文名稱：人70kDa熱休克蛋白5(HSPA5)測(cè)定試劑盒
本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中人70kDa熱休克蛋白5(HSPA5)測(cè)定試劑盒水平。用純化的抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入，再與HRP標(biāo)記的結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中的濃度。
安全－無酚等危險(xiǎn)的有機(jī)物抽提可靠－操作簡(jiǎn)單，每次都能獲得理想的效果快速－可在35分鐘內(nèi)完成一次抽提工作方便－用小型柱就可以獲得較大量的質(zhì)粒DNA。
The E-Z 96? Mag-Bind? Tissue RNA Kit provides a novel technology for total RNA isolation from plant and animal tissues. This kit allows the rapid and reliable isolation of high-quality total cellular RNA from a wide variety of tissues. Samples are first lysed in a specially formulated buffer and then proteins and DNA are precipitated. The Supertanent is combined with magentic beads and after a couple of quick wash steps pure RNA is eluted. Unlike column-based systems, the binding of nucleic acids to magnetic partciles occurs in solution, resulting in increased binding kinetics and binding efficiency. Particles are also completly re-suspended during the wash steps of the purification protocol, which enhances contact with the wash buffer and removal of contaminants and increasing nucleic acid purity. E.Z.N.A.? Mag-Bind? Tissue RNA procedure can be automated with most robotic workstations。
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