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北京方程嘉鴻科技有限公司
北京方程嘉鴻科技有限公司
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更新時間：2017-04-11 20:49:42瀏覽次數(shù)：266次
聯(lián)系我時，請告知來自 智慧城市網(wǎng)北京方程生物公司經(jīng)營銷售T細(xì)胞特異性鳥苷三磷酸酶（Tgtp）ELISA試劑盒，可以提供免費代測，送檢贈京東卡活動正在進(jìn)行中，詳情咨詢銷售人員
AP12ELISA Ki
試劑盒名稱:T細(xì)胞特異性鳥苷三磷酸酶(Tgtp)ELISA試劑盒
英文名：Human apelin 12,AP12
品牌:BIOFINE
種屬:大鼠ELISA試劑盒
檢測波長:450nm
所需樣本體積: 50-100ul
適用范圍:僅供科研
保存及有效期:2-8℃，六個月，-20℃一年
檢測目的:用于測定血清，血漿及相關(guān)液體T細(xì)胞特異性鳥苷三磷酸酶(Tgtp)含量。適合檢測包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等標(biāo)本。
Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.
T細(xì)胞特異性鳥苷三磷酸酶(Tgtp)另一片段類似Fc，隨后被分解成小分子多肽，無生物活性。 IgM是由五個單體組成的五聚體，含10個重鏈和10個輕鏈，具有10個抗原結(jié)合價，由于空間位置的影響，只表現(xiàn)為五個抗原結(jié)合價。IgM分子量約為900000，IgG分子量約為150000。 機(jī)體被微生物感染后，先產(chǎn)生IgM抗體，然后產(chǎn)生IgG抗體。經(jīng)過一段時間，IgM抗體量逐漸減少而消失，而IgG抗體可*存在，在疾病*后可持續(xù)數(shù)年之久。IgM抗體一般為保護(hù)性抗體，具有免疫性。因此IgM抗體的測定，對某些傳染病如甲型肝炎有較高的臨床診斷價值。
T細(xì)胞特異性鳥苷三磷酸酶(Tgtp)北京
活化蛋白C(APC)
乙胺碘呋酮(AD)
磷酯酰肌醇特異性磷酯酶C(PIPLC)
血纖肽/纖維蛋白肽A(FPA)
可溶性CD40配體(sCD40L)
細(xì)胞凋亡抑制因子(IAP)
基質(zhì)γ羧基*蛋白(MGP)
腫瘤特異性抗原(TSA)
彈性蛋白(ELN)
生長調(diào)節(jié)致癌基因α/黑素瘤生長ci激因子(GROα/CXCL1/MGSA)
大內(nèi)皮素(Big ET)
神經(jīng)細(xì)胞粘附分子配體1(NCAM-L1/CD171)
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