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北京方程嘉鴻科技有限公司


當(dāng)前位置:北京方程嘉鴻科技有限公司>>ELISA試劑盒>>大鼠ELISA試劑盒>>抗心磷脂抗體IgG(ACA-IgG)

抗心磷脂抗體IgG(ACA-IgG)

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北京方程生物公司經(jīng)營銷售抗心磷脂抗體IgG(ACA-IgG)ELISA試劑盒,可以提供免費代測,送檢贈京東卡活動正在進行中,詳情咨詢銷售人員

詳細(xì)介紹

ELISA是以免疫學(xué)反應(yīng)為基礎(chǔ),將抗原、抗體體的特異性反應(yīng)與酶對底物的高效催化作用相結(jié)合起來的一種敏感性很高的試驗技術(shù)。

抗心磷脂抗體IgG(ACA-IgG)是北京方程生物公司的優(yōu)勢產(chǎn)品,2017年買抗心磷脂抗體IgG(ACA-IgG)送好禮*活動火熱進行中......

 

抗心磷脂抗體IgG(ACA-IgG)

黑色素細(xì)胞抗體

 

Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.

 

抗心磷脂抗體IgG(ACA-IgG)elisa試劑盒回收率是反應(yīng)待測物在樣品分析過程中的損失的程度,損失越少,回收率越高,如果作標(biāo)液1PPM,就是1毫克/升,而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升,就是說你的回收率是99%,這個與真實成分有密切的關(guān)系,說明方法的準(zhǔn)確度。比如水中總無機氯含量測定,樣品水中含有無機氯20mg/L,取100mL被測水樣品,加入0.1mL濃度為10mg/mL的含無機氯標(biāo)準(zhǔn)樣品,測定時忽略體積變化,如果測定出樣品中無機氯為2.98mg/L,則認(rèn)為回收率為99%。

 

抗心磷脂抗體IgG(ACA-IgG)

白三烯D4(LTD4)

粒細(xì)胞集落刺激因子(G-CSF)

血管生成素受體/含免疫球蛋白樣環(huán)和上皮生長因子樣域*激酶2(Tie-2)

抗胸腺細(xì)胞球蛋白(ATG)

網(wǎng)膜素(omentin)

橫紋肌輔肌動蛋白α (sm Actinin-α )

硫酸角質(zhì)素(KS)

酰化刺激蛋白(ASP)

骨堿性磷酸酶(BALP)

細(xì)胞纖維連接蛋白(cFn)

庚型肝炎病毒IgM(HGV-IgM)

細(xì)胞毒素相關(guān)蛋白A(CagA)

 

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