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北京方程嘉鴻科技有限公司
北京方程嘉鴻科技有限公司
北京方程生物公司經營銷售免疫核糖核酸（Irna）ELISA試劑盒，可以提供免費代測，送檢贈京東卡活動正在進行中，詳情咨詢銷售人員
ER
試劑盒名稱:免疫核糖核酸(Irna)ELISA試劑盒
英文名：Human Estradiol receptor,ER
品牌:BIOFINE
種屬:大鼠ELISA試劑盒
檢測波長:450nm
所需樣本體積: 50-100ul
適用范圍:僅供科研
保存及有效期:2-8℃，六個月，-20℃一年
檢測目的:用于測定血清，血漿及相關液體免疫核糖核酸(Irna)含量。適合檢測包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細胞培養上清、組織勻漿等標本。
ELISA的基礎是抗原或抗體的固相化及抗原或抗體的酶標記。結合在固相載體表面的抗原或抗體仍保持其免疫學活性，酶標記的抗原或抗體既保留其免疫學活性，又保留酶的活性。在測定時，受檢標本（測定其中的抗體或抗原）與固相載體表面的抗原或抗體起反應。用洗滌的方法使固相載體上形成的抗原抗體復合物與液體中的其他物質分開。再加入酶標記的抗原或抗體，也通過反應而結合在固相載體上。此時固相上的酶量與標本中受檢物質的量呈一定的比例。加入酶反應的底物后，底物被酶催化成為有色產物，產物的量與標本中受檢物質的量直接相關，故可根據呈色的深淺進行定性或定量分析。由于酶的催化效率很高，間接地放大了免疫反應的結果，使測定方法達到很高的敏感度。
免疫核糖核酸(Irna)Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.
免疫核糖核酸(Irna)價格
不耐熱腸毒素B亞單位(LTB)
尿游離*(UFC)
Ⅱ型膠原螺旋肽(HELIX-Ⅱ)
骨成型蛋白7(BMP-7)
*(FA)
卵泡抑素(FS)
真核翻譯起始因子3a(eIF3a)
促甲狀腺素(TSH)
沙眼衣原體抗體(CT)
超敏生長激素(U S-GH)
高鐵血紅蛋白(MHB)
系統性紅斑狼瘡(SLE)
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