艾美捷人β2微球蛋白英文說明書：

INTRODUCTION

Beta 2 microglobulin is an 11 kDa protein associated with the outer membrane of many cells including lymphocytes. It is the small subunit of the MHC class I molecule. Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Beta 2 microglobulin associates with class I-like molecules such as CD1 and Qa as well as with the alpha chain of MHC class I molecules. Very limited amounts of MHC class I molecules can be found on the surface in the absence of Beta 2 microglobulin. CD8 T cells cannot develop in the absence of MHC class I.

Beta 2-microglobulin is present in small amounts in serum, csf, and urine of normal people, and to a much greater degree in the urine and plasma of patients with tubular proteinaemia, renal failure, or kidney transplants. Human Beta 2 microglobulin levels can rise either because its rate of synthesis has increased (e.g. in AIDS, malignant monoclonal plasma cell dyscrasia, solid tumors and autoimmune disease) or because of impaired renal filtration (e.g. due to renal insufficiency, graft rejection or nephrotoxicity induced by post-transplantation immunosuppressive therapy). Beta-2 microglobulin levels might also be elevated in multiple myeloma and lymphoma cases. Dialysis-related amyloidosis develops after a long-term hemodialysis, it can aggregate into amyloid fibers that deposit in joint spaces.

DESCRIPTION

Human Beta-2 Microglobulin produced in Human urine from patients with tubular proteinuria having a molecular mass of 12KDa.

SOURCE

Human urine from patients with tubular proteinuria.

PHYSICAL APPEARANCE

Sterile Filtered White lyophilized (freeze-dried) powder.

FORMULATION

Lyophilized from 0.02M NH4HCO3.

SOLUBILITY

It is recommended to reconstitute the lyophilized HuB2M in phosphate buffer pH > 7.0 containing 0.15M NaCl.

STABILITY

Human B2M although stable at room temperature for 3 weeks, should be stored between 2-8°C.

PURITY

Greater than 98.0%.

HUMAN VIRUS TEST

Starting material tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, Hepatitis C antibodies and Syphilis.

SAFETY DATA SHEET

SDS

USAGE

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

艾美捷人β2微球蛋白中文說明書：

人β2微球蛋白是一種11kDa蛋白，與包括淋巴細胞在內(nèi)的許多細胞的外膜相關(guān)。它是MHC I類分子的小亞單位。與β2-微球蛋白結(jié)合通常是I類重鏈從內(nèi)質(zhì)網(wǎng)運輸?shù)郊毎砻嫠匦璧?/span>人β2微球蛋白與I類分子如CD1和Qa以及MHC I類分子的α鏈相關(guān)。在沒有人β2微球蛋白。CD8 T細胞在沒有MHC I類的情況下不能發(fā)育。

β2-微球蛋白在正常人的血清、腦脊液和尿液中少量存在，在腎小管蛋白血癥、腎衰竭或腎移植患者的尿液和血漿中的含量更高。人β2微球蛋白水平可能會升高，因為其合成速度加快（例如在艾滋病、惡性單克隆漿細胞疾病、實體瘤和自身免疫性疾病中），或者因為腎過濾功能受損（例如由于腎功能不全、移植排斥或移植后免疫抑制治療引起的腎毒性）。在多發(fā)性骨髓瘤和淋巴瘤患者中，β-2微球蛋白水平也可能升高。透析相關(guān)淀粉樣變在長期血液透析后發(fā)生，它可以聚集成淀粉樣纖維沉積在關(guān)節(jié)間隙。

艾美捷人β2微球蛋白化學(xué)性質(zhì)：

編號：PRO-553

同義詞：Beta-2-微球蛋白，B2M。

資源：管狀蛋白尿患者的人尿。

形態(tài)：無菌過濾的白色凍干（冷凍干燥）粉末。

公式：從 0.02M NH4HCO3 凍干。

溶解度：建議在含有 0.15M NaCl 的磷酸鹽緩沖液 pH > 7.0 中重構(gòu)凍干的 HuB2M。

穩(wěn)定：人 B2M 雖然在室溫下可穩(wěn)定保存 3 周，但應(yīng)在 2-8°C 之間儲存。

純度：大于 98.0%。

人類病毒測試：對起始材料進行測試，發(fā)現(xiàn) HIV I 和 II 抗體、乙型肝炎表面抗原、丙型肝炎抗體和梅毒呈陰性。

僅供實驗室研究使用。不得用作藥物、農(nóng)業(yè)或農(nóng)藥產(chǎn)品、食品添加劑或家用化學(xué)品。