艾美捷支原體檢測試劑盒英文說明：

The SouthernBiotech Mycoplasma Detection Kit is designed to specifically detect potential ycoplasma contamination in cell cultures.The kit incorporates polymerase chain reaction (PCR) to amplify the conserved 16S ribosomal RNA coding region within the Mycoplasmagenome, thereby providing an extensive, highly sensitive, and efficient detection method. Carefully determined primer sequences cover three genera of Mycoplasmatales (Mycoplasma, Acholeplasma, and Ureaplasma) which allows the kit to detect over 95% of potential cell culture infections.

The PCR technology in the Mycoplasma Detection Kit is fast (results are typically obtained in less than 3 hours) and easy to use. The kit is also highly sensitive and can detect as little as 2-5 femtograms of Mycoplasma DNA in 100 µL of test sample supernatant. Eukaryotic and bacterial DNA from cell  ulture supernatant is not amplified by the kit.

A sample cell line infected with Mycoplasma will generate a PCR product between ~448 bp to ~611 bp on an agarose gel, depending on the type of Mycoplasma present. A positive control (M. orale, 503 bp) is included to validate that the PCR amplification process has occurred as well as to confirm the size of the PCR product obtained in test samples. An internal control is also provided to eliminate

potential false negatives associated with PCR inhibitors.

Kit Components：

• User guide

• Primer set and nucleotides (lyophilized) - Blue Cap

o Primer/dNTP mix (deoxynucleotide triphosphates including dATP, dCTP, dGTP, and dUTP)

• Sterile PCR 10X reaction buffer (500 μL) - White Cap

o 100 mM Tris-HCl, pH 8.5

o 750 mM KCl

o 30 mM MgCl2

• Positive Control DNA (lyophilized) - Yellow Cap

o Non-infectious PCR product from M. orale

o Yields a 503 bp band

• Internal Control DNA (lyophilized) - Green Cap

o Non-infectious plasmid DNA including Mycoplasma-specific primer sequences

o Yields a 270 bp band

艾美捷支原體檢測試劑盒中文說明：

組成：操作手冊，設計引物/核苷酸Mix（dATP、dCTP、dGTP、dTTP），凍干粉，藍蓋裝； 無菌PCR反應緩沖液10X，500uL，白蓋裝；陽性對照DNA（非傳染性），凍干粉，黃蓋裝；內控對照質粒DNA（非傳染性），凍干粉，綠蓋裝；試劑盒不含PCR儀，DNATag酶，PCR反應管，無DNA水

艾美捷支原體檢測試劑盒特點：

1、試劑盒通過擴增支原體基因組中高度保守的16SrRNA編碼區序列，實現培養細胞是否有支原體污染的檢測

2、設計引物中含有支原體、無膽甾原體、尿素原體，能檢測出95%以上的感染細胞；試劑盒操作簡便，3小時內可出實驗結果，靈敏性高，可以檢測100uL樣品上清液中2。5毫微微克級的DNA，對于真核和細菌DNA不產生擴增效應

3、試劑盒提供的陽性對照在DNA電泳上會顯示503bp的條帶，提供的內控對照在DNA電泳上會顯示270bp的條帶（內控對照是PCR反應成功的標志）

相關文獻：

1. Crittenden MR, Zebertavage L, Kramer G, Bambina S, Friedman D, Troesch V, et al. Tumor cure by radiation therapy and checkpoint inhibitors depends on pre-existing immunity. Sci

Rep. 2018;8:7012.

2. Razidlo GL, Burton KM, McNiven MA. Interleukin-6 promotes pancreatic cancer cell migration by rapidly activating the small GTPase CDC42. J Biol Chem. 2018;293:11143-53.

3. Pishas KI, Drenberg CD, Taslim C, Theisen ER, Johnson KM, Saund RS, et al. Therapeutic targeting of KDM1A/LSD1 in Ewing sarcoma with SP-2509 engages the endoplasmic

reticulum stress response. Mol Cancer Ther. 2018;17:1902-16.

4. Bryant JD, Sweeney SR, Sentandreu E, Shin M, Ipas H, Xhemalce B, et al. Deletion of the neural tube defect-associated gene Mthfd1l disrupts one-carbon and central energy

metabolism in mouse embryos. J Biol Chem. 2018;293:5821-33.