Product Details

The RMP1-14-CP151 monoclonal antibody is a chimeric version of the original RMP1-14 antibody. The variable domain sequences are identical to the original RMP1-14 but the constant region sequences have been switched from rat IgG2a to mouse IgG2a. The RMP1-14-CP151 antibody also contains a D265A mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. RMP1-14-CP151 reacts with mouse PD-1 (programmed death-1) also known as CD279. PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. PD-1 expression declines after successful elimination of antigen. Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. PD-1’s structure includes a ITIM (immunoreceptor tyrosine-based inhibitory motif) suggesting that PD-1 negatively regulates TCR signals. PD-1 signals via binding its two ligands, PD-L1 and PD-L2 both members of the B7 family. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, and T-cell death. Additionally, PD-1 is known to play key roles in peripheral tolerance and prevention of autoimmune disease in mice as PD-1 knockout animals show dilated cardiomyopathy, splenomegaly, and loss of peripheral tolerance. Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, and breast adenocarcinoma. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and its receptor PD-1. For these reasons anti-PD-1 mediated immunotherapies are currently being explored as cancer treatments.

BioXCell RecombiMAb小鼠PD-1(CD279)抗體(D265A)：

RMP1-14-CP151單克隆抗體是原始RMP1-14抗體的嵌合版本。可變結構域序列與原始RMP1-14相同，但恒定區域序列已從大鼠IgG2a切換到小鼠IgG2a。RMP1-14-CP151抗體在Fc片段中還含有D265A突變，使其無法與內源性Fcγ受體結合。RMP1-14-CP151與小鼠PD-1（程序性死亡-1）反應，也稱為CD279。PD-1是由Pdcd50基因編碼的55-1 kDa細胞表面受體，屬于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺細胞以及活化的T和B淋巴細胞和骨髓細胞上短暫表達。PD-1在成功消除抗原后表達下降。

BioXCell艾美捷RecombiMAb小鼠PD-1(CD279)抗體(D265A)規格：

#  BXC-CP151

Isotype Mouse IgG2a, κ

Recommended Isotype Control(s) RecombiMAb mouse IgG2a (D265A) isotype control, anti-hen egg lysozyme

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Mutations D265A

Immunogen Syrian Hamster BKH cells transfected with mouse PD-1 cDNA

Reported Applications in vivo blocking of PD-1/PD-L signaling*

*Reported for the original rat IgG2a RMP1-14 antibody

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <1EU/mg (<0.001EU/μg)

Determined by LAL gel clotting assay

Aggregation <5%

Determined by DLS

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 μM filtered

Production Purified from CHO cell supernatant in an animal free facility

Purification Protein G

RRID AB_2927525

Molecular Weight 150 kDa

Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

同種型 小鼠IgG2a， κ

推薦的同種型對照 RecombiMAb小鼠IgG2a（D265A）同種型對照，抗母蛋溶-菌-酶

推薦的稀釋緩沖液 體內純 pH 7.0 稀釋緩沖液

突變 D265A

免疫原 用小鼠PD-1 cDNA轉染的敘利亞倉鼠BKH細胞

報告的應用程序 體內阻斷PD-1/PD-L信號傳導**

報道了原始大鼠IgG2a RMP1-14抗體

配方 PBS，pH 7.0

不含穩定劑或防腐劑

內毒素 <1EU/mg （<0.001EU/μg）

通過LAL凝膠凝血測定法測定

集合體 <5%

由 DLS 確定

純度 >95%

由 SDS-PAGE 決定

無 菌 0.2 μM 過濾

生產 從無動物設施中的CHO細胞上清液中純化

純化 蛋白 G

里德 AB_2927525

分子量 150 千達

鼠病原體檢測 外切粒菌/鼠痘病毒： 陰性 漢坦病毒： 陰性 K 病毒： 陰性乳酸脫氫酶升高病毒： 陰性 淋巴細胞性脈絡叢腦膜炎病毒： 陰性小鼠腺病毒： 陰性小鼠巨細胞病毒： 陰性小鼠肝炎病毒： 陰性小鼠分鐘病毒： 陰性小鼠諾如病毒： 陰性小鼠細小病毒： 陰性

小鼠輪狀病毒：肺支原體陰性：陰性 小鼠肺炎病毒：陰性 多瘤病毒：陰性呼腸孤病毒篩查：陰性 仙臺病毒：陰性 泰勒鼠腦脊髓炎：陰性

存儲 抗體溶液應以4°C的儲備濃度儲存。 不要凍結。