當前位置:武漢艾美捷科技有限公司>>技術文章>>中英文說明丨艾美捷木葡聚糖(克隆LM25)
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Xyloglucan is a hemiceullose or polysaccharide that can be found in the primary cell wall.
Product Information:
Immunogen Neoglycoprotein, Xyloglucan
Host Rat
Clonality Monoclonal
Subclass/isotype IgM
Purity Cell culture supernatant.
Format Liquid
Quantity 1 ml
Storage Store at +4°C (short term) and at -20°C (long term).
Tested applications ELISA (ELISA), Immuniflourescence (IF)
Recommended dilution 1:10 (ELISA, IF)
艾美捷木葡聚糖(克隆LM25)#AS18-4205-1ml詳細信息:
免疫原:新糖蛋白,木葡糖聚糖
宿主:大鼠
單克隆:克隆
亞類/同種型:IgM
純度:細胞培養上清液。
形態:液體
數量:1毫升
儲存溫度:為+4°C(短期)和-20°C(長期)。
測試應用:ELISA(ELISA)、免疫熒光(IF)
推薦稀釋度:1:10(ELISA,IF)
已確認的反應性:高等植物、蕨類植物和苔蘚
無反應性:目前還不知道預測反應性的已確認例外情況
Agrisera木葡聚糖(克隆LM25)應用示例:
Material: Cassava leaf, epidermal layer
Fixation: aldehyde, embedded in HM20 resin, thin sectioned
Blocking: 4% milk in PBS
Primary antibody: Anti-xyloglucan, LM25 (AS18 4205-1ml)
Secondary antibody (Agrisera): Donkey anti-Rat IgG (H&L), DyLight® 594 conjugated, AS12 2090 (red)
Counterstained with Calcofluor White (blue). Super-resolution images were acquired on a ZEISS Elyra7 in Lattice Structured Illumination Microscopy mode.
Courtesy Dr. Kirk Czymmek, Donald Danforth Plant Science Center, USA
Agrisera木葡聚糖(克隆LM25)文獻參考:
Pedersen et a. (2012). Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research. J Biol Chem. 2012 Nov 6;287(47):39429-38.doi: 10.1074/jbc.M112.396598.
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