Synthesis and structure

Antonia Red™ dextrans are synthesised from well-characterized dextran fractions derived from Leuconostoc mesenteroides labelled with Antonia Red. After purification from non-bound dye, the products are controlled for Mw, appearance, solubility, DS, fluorescence and free dye. The products are designated by the approximate molecular weights of the dextran fractions used. Thus, for example, the product Antonia Red dextran 4 has a molecular weight of approx. 4000 Da. The actual molecular weight is determined by GPC. This value is supplied with the Certificate of Analysis. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear α-(1-6)-linked glucose chain with however a low percentage (2-5%) of α-(1-3) branches distributed along the chain. The dextran fractions used are from Mw of 4000 to 150000 and are carefully controlled by GPC, optical rotation, absorbance and other control parameters.

Antonia Red-葡聚糖結(jié)構(gòu)特征

Spectral properties

Antonia Red dextran has an absorbance maximum at 583 nm (in borate buffer, pH 9.0) and an emission wavelength of 602 nm. The dye residue exhibits high photo- and chemical stability and bright fluorescence. The DS lies between 0.001-0.01.

Stability

No prospective stability studies on Antonia Red-dextrans have been performed. However, the structural properties of the dextran carrier and those of the chemical linkage of the Antonia Red-dye to the dextran chain would suggest good stability of the product. It is recommended that the products are stored in air-tight containers in the dark. Antonia Red dextrans may be stored at ambient temperatures.

Applications

Antonia Red dextrans are primarily used for studying permeability and transport in cells and tissues, trace cell lineage and perform vasculature studies. An added benefit is that measurements of the fluorescence provide quantitative data on the permeability of healthy and diseased tissues. Such studies can be performed in real time by intra-vital fluorescence microscopy. The technique offers high sensitivity and concentrations down to 1μg/ml can be detected in tissue fluids. Antonia Red dextrans is brightly fluorescent using the red (Texas Red) filter in fluorescence microscopy

艾美捷Antonia Red-葡聚糖#ARD4/ARD20/ARD40/ARD150, 為紫色粉末，易溶于水或電解質(zhì)溶液。它們主要用于研究細(xì)胞和組織中的滲透性和轉(zhuǎn)運(yùn)、細(xì)胞譜系示蹤和脈管系統(tǒng)研究。

Antonia Red-葡聚糖是由來(lái)源于用Antonia紅標(biāo)記的間質(zhì)明串珠菌。從非結(jié)合染料中純化后控制產(chǎn)品的分子量、外觀、溶解度、DS、熒光和游離染料。產(chǎn)品由所用葡聚糖級(jí)分的近似分子量表示。因此，對(duì)于例如，產(chǎn)品Antonia Red右旋糖酐4的分子量約為4000Da

分子量通過(guò)GPC測(cè)定。此值隨分析證書(shū)一起提供。這個(gè)所用的右旋糖酐來(lái)自間質(zhì)明串珠菌B-512F，其本質(zhì)上是線性α-（1-6）連接的然而，沿鏈分布的α-（1-3）分支的百分比較低（2-5%）。所使用的葡聚糖級(jí)分的Mw為4000至150000，并通過(guò)GPC、光學(xué)旋轉(zhuǎn)、吸光度和其他控制參數(shù)。

應(yīng)用：

Antonia Red-葡聚糖主要用于研究細(xì)胞和組織中的滲透性和轉(zhuǎn)運(yùn)，追蹤細(xì)胞譜系并進(jìn)行脈管系統(tǒng)研究。另一個(gè)好處是熒光提供了關(guān)于健康和患病組織的滲透性的定量數(shù)據(jù)。這樣的研究可以通過(guò)活體內(nèi)熒光顯微鏡實(shí)時(shí)進(jìn)行。該技術(shù)提供在組織液中可以檢測(cè)到高靈敏度和低至1μg/ml的濃度。安東尼婭·雷德在熒光顯微鏡中使用紅色（得克薩斯紅）濾光片，葡聚糖具有明亮的熒光。

Antonia Red-葡聚糖相關(guān)研究：

ARD4 Antonia Red-dextran 4 4000 10mg

ARD4 Antonia Red-dextran 4 4000 50mg

ARD20 Antonia Red-dextran 20 20000 10mg

ARD20 Antonia Red-dextran 20 20000 50mg

ARD40 Antonia Red-dextran 40 40000 10mg

ARD40 Antonia Red-dextran 40 40000 50mg

ARD150 Antonia Red-dextran 150 150000 10mg

ARD150 Antonia Red-dextran 150 150000 50mg