丙二醛(MDA)elisa檢測試劑盒英文介紹：

Principle of the Assay：

This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are wash ed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

Precautions

1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot experiment using standards and a small number of samples is recommended.

2. After opening and before using, keep plate dry.

3. Before using the kit, spin tubes and bring down all components to the bottom of tubes.

4. Storage TMB reagents avoid light.

5. Washing process is very important, not fully wash easily cause a false positive and high background.

6. Duplicate well assay is recommended for both standard and sample testing.

7. Don’t let microplate dry at the assay, for dry plate will inactivate active components on plate.

8. Don’t reuse tips and tubes to avoid cross contamination.

9. Please do not mix the reagents in different kits of our company. Do not mix reagents from other manufacturers.

10. To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.

艾美捷丙二醛(MDA)elisa檢測試劑盒#EKF60157檢測原理：

該試劑盒基于競爭ELISA檢測方法。該試劑盒中提供的微量滴定板已預先涂有目標在反應過程中，樣品或標準品中的靶標與固相載體上固定量的靶標競爭用于靶特異性的生-物-素化檢測抗體上的位點。洗滌過量的綴合物和未結合的樣品或標準品并將HRP鏈親和素（SABC）加入每個微孔板孔中并孵育。然后TMB基板溶液添加到每個井中。通過加入硫酸溶液和顏色變化終止酶底物反應在450nm的波長下用分光光度法測量。然后通過以下方式確定樣品中目標的濃度將樣品的OD與標準曲線進行比較。

丙二醛(MDA)elisa檢測試劑盒操作步驟：

步驟1：在預涂板上分別設置標準、試樣、對照（空白）孔，然后記錄它們的位置。

第2步：在每個孔中加入50ul標準品或樣品。立即向每個孔中加入50ul生-物-素標記的抗體，輕輕敲擊

平板以確保充分混合，然后在37°C下孵育45分鐘。

清洗步驟：抽吸并清洗盤子3次。

步驟3：向每個孔中加入100ul SABC工作溶液，并在37°C下孵育30分鐘。

清洗步驟：抽吸并清洗盤子5次。

第4步：添加90ul TMB基質溶液。在37°C下培養10-20分鐘。

步驟5：添加50ul停止溶液。在450nm下立即讀取并計算

注意事項：

1.為了檢驗實驗操作的有效性和樣品稀釋比例的適當性，建議使用標準品和少量樣品進行中試。

2.打開后和使用前，請保持盤子干燥。

3.在使用試劑盒之前，旋轉試管，并將所有組件降到試管底部。

4.TMB試劑應避光保存。

5.洗滌過程非常重要，不完-全洗滌容易造成假陽性和高背景。

6.對于標準測試和樣品測試，建議使用雙孔分析法。

7.在測定時不要讓微孔板干燥，因為干燥的平板會使平板上的活性成分失活。

8.不要重復使用尖-端和管道，以避免交叉污染。

9.請不要將試劑混合在我們公司的不同試劑盒中。不要混合其他制造商的試劑。

10.為了確保準確的結果，在培養步驟中需要適當地粘附平板密封劑。