PolyMag CRISPR kit is the only magnet assisted transfection reagent optimized to deliver high level of plasmid DNA and/or mRNA expressing Cas9 and guide RNA (gRNA). This reagent is based on our proprietary Magnetofection™ technology. The nanoparticles are composed of iron oxide magnetic core coated with cationic molecules. For your gene editing experiments, this reagent provides high transfection efficiency without toxicity.

Benefits:

•High efficiency delivery of CRISPR/Cas9 system even on primary and hard to transfect cells

•Ideal for co-transfection of pDNA/pDNA, pDNA/gRNA, pDNA/mRNA

•Optimized for large plasmid DNA containing Cas9, and guide RNA

•Low cell toxicity - enable to start your experiment with less cell

艾美捷POLYMAG CRISPR 200 轉染試劑#PNC40200是經過優化的磁鐵輔助轉染試劑，可提供表達 Cas9 和向導 RNA （gRNA） 的高水平質粒 DNA 和/或 mRNA。該試劑基于我們專有的磁感應™技術。納米顆粒由涂有陽離子分子的氧化鐵磁芯組成。對于您的基因編輯實驗，該試劑具有高轉染效率，無毒性。

POLYMAG CRISPR 200 轉染試劑特點：

• 即使在原代細胞和難以轉染的細胞上也能高效遞送 CRISPR/Cas9 系統• 非常適合共轉染 pDNA/pDNA、pDNA/gRNA、pDNA/mRNA

• 針對含有 Cas9 的大質粒 DNA 進行了優化，并引導 RNA

• 低細胞毒性 - 能夠以更少的細胞開始實驗

POLYMAG CRISPR 200 轉染試劑結果示例：

圖：PolyMag CRISPR共轉染效率用于CRISPR-Cas9實驗。將NIH-3t3、HeLa和MDCK細胞接種在完-全培養基中的24孔板中，轉染時最佳密度為50-70%匯合。用GFP-Cas9質粒和RFP pDNA轉染細胞，然后在37oC下孵育過夜。轉染后24h熒光顯微鏡監測GFP和RFP表達。