Normal serum is used to describe the serum from animals that have not been immunized with specific antigens to produce antibodies. Therefore, normal serum is opposite to antiserum. Normal serum contains general complement of serum proteins, including different kinds of immunoglobulins that exist in healthy animals of specific species. The normal serum products provided are extracted with lipids to improve transparency, and desalted and dialyzed to remove phosphate buffer containing sodium azide, And finally freeze-dried to obtain freeze-dried powder. Normal serum is very suitable as a blocking reagent to remove non-specific hybridization. Normal serum is often used as a control when detecting general or specific antibody purification methods.

Amyjet normal goat serum # 005-000-121, which comes from a non immune goat host, is purified by lipid extraction and dialysis with PBS solution containing sodium azide to improve its transparency. It is recommended to use PBS to dilute the sealed serum stock solution 5-20 times, prepare it into a working solution of 5% -20%, and directly drip it onto tissue slices or cell smears. Incubate at 37 ℃ for 10 to 30 minutes, remove the serum, do not wash it, and drip an appropriate proportion of diluted primary antibody working solution. Incubate at 37 ℃ for 1-2 hours or 4 ℃ overnight. Note: The serum used for blocking cannot contain the target protein and is different from the source of the first antibody. Blocking serum from the same source as the second antibody can be used.

正常血清是用來描述來自沒有用特異抗原進行免疫產生抗體的動物的血清.因此，正常血清與抗血清相對.正常血清包含血清蛋白的一般補體，包括存在于特定物種健康動物體內的不同種類的免疫球蛋白.提供的正常血清產品經過脂類抽提以提高透明度，經去鹽和透析處理以去除包含疊-氮-化-鈉的磷酸緩沖液，并最后凍干以獲得凍干粉末.正常血清非常適用于作為封閉試劑去除非特異性雜交.正常血清在檢測一般或特異抗體純化方法的時候也經常作為對照使用.

艾美捷正常山羊血清#005-000-121，來源于非免疫的山羊宿主，經過脂質萃取和含疊-氮-化-鈉的PBS溶液透析，改善其透明度。推薦用PBS將封閉血清原液稀釋5-20倍，配成5%-20%的工作液，直接滴加在組織切片或者細胞涂片上，37℃度孵育10-30分鐘，清除血清，勿洗，滴加適當比例稀釋的一抗工作液，37℃孵育1-2h或4℃過夜。注意：封閉用血清中不能含有目標蛋白，且與一抗來源不同，可用與二抗相同來源的封閉血清。

正常山羊血清#005-000-121基本參數：

中文名稱：正常山羊血清

英文名字：Normal Goat Serum

編號：005-000-121

規格：10ml

物理狀態：凍干固體

建議工作濃度或稀釋范圍：

出于封閉目的，使用5%（v/v）溶液（從再水合體積中稀釋1:20）。按照說明重新補水可產生100%的血清。

描述：該正常山羊血清產品可應用于大多數免疫學實驗，用作封閉液或空白對照.

保存建議：凍干粉保存在2-8°C，準備用時，根據指示的體積加入雙蒸水，在2-8°C條件下，未稀釋的液體保質期6周，每天準備新鮮的工作液.如果重溶后，想延長保存期，將未稀釋的溶液保存在-20°C或者更低，避免反復凍融.