背景資料

Recombinant Mouse Sonic Hedgehog is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. 3 mammalian hedgehog genes （sonic，desert，Indian） share about 60% homology. The Mouse Sonic Hedgehog is 99% homologous to the human gene. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube，the anterior-posterior limb axis，and the ventral somites. Sonic HedgeHog binds to the patched receptor，which functions in association with smoothened，to activate the transcription of target genes. In the absence of sonic HedgeHog，patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor，the gli oncogene. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites，and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog exhibits both floor plate- and motor neuron-inducing activity. Mutations in a long-range Sonic HedgeHog enhancer located in an intron of the limb region 1 gene result in preaxial polydactyly.

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來源宿主

大腸桿菌中重組表達

溶解建議

提供的小鼠音猬因子（mSHH）產品為過濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml小鼠音猬因子（mSHH）的10mM磷酸鈉緩沖液（pH 7.5）中凍干產生的.我們建議加無菌的18MΩ-cm水以配制工作儲存液到0.1-0.5mg/ml.

保存建議

小鼠音猬因子（mSHH）在室溫可保存3周，或置于-18℃凍存用于長期保存.重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長保存時間，建議分裝后置于-18℃凍存.請避免反復凍融.

其他

ProSpec 是一家全球著名細胞因子蛋白生產和研發的公司，具有20多年的生產經驗，專注于蛋白（重組及合成）生產研發。我們的核心產品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 的生產工藝和規模使其可以提供毫克到克級蛋白，價格優于同類公司。絕大部分產品是天然成熟型蛋白，而不含有標簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產品沒有添加保護劑或鹽，這就對工藝提出了更高的要求。同時，ProSpec-Tany絕大部分產品為凍干粉，因此易于運輸和保存。