產品新聞

背景資料

High-mobility group box 1 protein （HMGB1）， previously known as HMG-1 or amphoterin， is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa， 215 amino acid （aa） single chain polypeptide containing three domains: two N-terminal globular， 70 aa positively charged DNA-binding domains （HMG boxes A and B）， and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4， 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported， with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.

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來源宿主

大腸桿菌中重組表達

溶解建議

Sterile Filtered White lyophilized （freeze-dried） powder. The HMG1 （1 mg/ml） was lyophilized after extensive dialyses against 1x PBS pH-7.4. It is recommended to reconstitute the lyophilized HMGB1 in sterile 18MΩ-cm H2O not less than 100 ug/ml， which can then be further diluted to other aqueous solutions.

保存建議

Lyophilized HMGB1 although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution HMGB1 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.

其他

ProSpec 是一家全球著名細胞因子蛋白生產和研發的公司，具有20多年的生產經驗，專注于蛋白（重組及合成）生產研發。我們的核心產品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。  的生產工藝和規模使其可以提供毫克到克級蛋白，價格優于同類公司。絕大部分產品是天然成熟型蛋白，而不含有標簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產品沒有添加保護劑或鹽，這就對工藝提出了更高的要求。同時，ProSpec-Tany絕大部分產品為凍干粉，因此易于運輸和保存。