中文名稱    信號調節(jié)蛋白α抗體   
英文名稱    SIRP Alpha      
信號調節(jié)蛋白α抗體    
研究領域    神經(jīng)生物學      
抗體來源    Rabbit    
克隆類型    Polyclonal    
交叉反應    Human, Mouse, Rat, Dog, Pig, Cow, Horse,     
產(chǎn)品應用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:50-300 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    
分 子 量    56kDa    
細胞定位    細胞膜     
性    狀    Lyophilized or Liquid    
濃    度    1mg/1ml    
免 疫 原    KLH conjugated synthetic peptide derived from human CD172a    
亞    型    IgG    
純化方法    affinity purified by Protein A    
儲 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    
保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 信號調節(jié)蛋白α抗體    

產(chǎn)品介紹    background:
Protein tyrosine phosphatases (PTPases) SHP1 and SHP2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1),SHPS1 , MyD1, BIT and p84. SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP2. SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.


處理標本：
（1）將標本以5%福爾馬林pH 7.2(4℃)PBS液固定40min～60min。
（2）用冷的PBS液洗滌，離心。
（3）如是組織塊，則在解剖顯微鏡下切成更小塊，放入試管中，加入鐵蛋白－抗體結合物置室溫20min，不時振蕩,
（4）以冷PBS液洗滌三次，離心。
（5）沉淀以2.5%戊二醛固定20min，以PBS洗滌。
（6）再以鋨酸固定，脫水包埋。也可以先超薄切片，再進行鐵蛋白－抗體結合物染色。

單抗的制備過程：
1.動物免疫：注射特定抗原蛋白
2.分離脾淋巴細胞：與培養(yǎng)好的骨髓瘤細胞混合，人工誘導細胞融合。
3.細胞融合：淋巴細胞雜交瘤的制備
4.雜交瘤細胞的篩選：HAT選擇培養(yǎng)基篩選出雜交瘤細胞。
5.培養(yǎng)雜交瘤細胞：體外培養(yǎng)雜交瘤細胞，從培養(yǎng)液中分離提取單克隆抗體。或者，將雜交瘤細胞移植到小鼠腹腔內(nèi)，從小鼠腹水中分離提取單克隆抗體。
6.單克隆抗體的制備和凍存、純化