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研域(上海)化學試劑有限公司
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大鼠Smad7 檢測試劑盒用純化的抗體包被微孔板,制成固相載體,TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成Z終的黃色。顏色的深淺和樣品中的待測物質呈正相關。用酶標儀在450nm波長下測定吸光度(OD值),計算樣品濃度。
大鼠Smad7 檢測試劑盒, ,別名: 大鼠Smad7 ELISA試劑盒, 大鼠Smad7 ELISA Kit 大鼠Smad7 檢測試劑盒,英文名: Smad7 ELISA Kit
完整的ELISA試劑盒包含以下各組分:
(1)已包被抗原或抗體的固相載體(免疫吸附劑);
(2)酶標記的抗原或抗體(結合物);
(3)酶的底物;
(4)陰性對照品和陽性對照品(定性測定中),大鼠Smad7 檢測試劑盒參考標準品和控制血清(定量測定中);
(5)結合物及標本的稀釋液;
(6)洗滌液,在板式ELISA中,常用的稀釋液為含0.05%吐溫20磷酸緩沖鹽水;
(7)酶反應終止液,常用的HRP反應終止液為硫酸,其濃度按加量及比色液的zui終體積而異,在板式ELISA中一般采用2mol/L。
Y149124 Goat Anti-rat IgG/PE PE標記的羊抗大鼠IgG 0.1ml
Y149125 Goat Anti-rat IgG/Cy7 Cy7標記的羊抗大鼠IgG 0.1ml
Y149126 Goat Anti-rat IgG/Cy5.5 Cy5.5標記的羊抗大鼠IgG 0.1ml
Y149127 Goat Anti-rat IgG/Cy5 Cy5標記的羊抗大鼠IgG 0.1ml
Y149128 Goat Anti-rat IgG/Cy3 Cy3標記的羊抗大鼠IgG 0.1ml
Y149129 Goat Anti-Mouse IgM/HRP 辣根過氧化物酶標記的羊抗小鼠IgM 0.1ml
Y149130 Goat Anti-Mouse IgM/Bio *標記的羊抗小鼠IgM 0.1ml
Y149131 Goat Anti-Mouse IgM/Gold 膠體金標記的羊抗小鼠IgM 0.5ml
Y149132 Goat Anti-Mouse IgM/AP 堿性磷酸酶(AP)標記的羊抗小鼠IgM 0.1ml
Y149133 Goat Anti-Mouse IgM/PE-Cy7 PE-Cy7標記的羊抗小鼠IgM 0.1ml
Y149134 Goat Anti-Mouse IgM/PE-Cy5.5 PE-Cy5.5標記的羊抗小鼠IgM 0.1ml
Y149135 Goat Anti-Mouse IgM/Alexa Fluor 647 Alexa Fluor 647標記的羊抗小鼠IgM 0.1ml
Y149136 Goat Anti-Mouse IgM/Alexa Fluor 555 Alexa Fluor 555標記的羊抗小鼠IgM 0.1ml
Y149137 Goat Anti-Mouse IgM/Alexa Fluor 488 Alexa Fluor 488標記的羊抗小鼠IgM 0.1ml
Y149138 Goat Anti-Mouse IgM/Alexa Fluor 350 Alexa Fluor 350標記的羊抗小鼠IgM 0.1ml
Y149139 Goat Anti-Mouse IgM/APC APC標記的羊抗小鼠IgM 0.1ml
Y149140 Goat Anti-Mouse IgM/PE-CY5 PE-CY5標記的羊抗小鼠IgM 0.1ml
Y149141 Goat Anti-Mouse IgM/PE-Cy3 PE-Cy3標記的羊抗小鼠IgM 0.1ml
Y149142 Goat Anti-Mouse IgM/PE PE標記的羊抗小鼠IgM 0.1ml
Y149143 Goat Anti-Mouse IgM/Cy7 Cy7標記的羊抗小鼠IgM 0.1ml
Y149144 Goat Anti-Mouse IgM/Cy5.5 Cy5.5標記的羊抗小鼠IgM 0.1ml
Y149145 Goat Anti-Mouse IgM/Cy5 Cy5標記的羊抗小鼠IgM 0.1ml
Y149146 Goat Anti-Mouse IgM/Cy3 Cy3標記的羊抗小鼠IgM 0.1ml
Y149147 Goat Anti-Mouse IgM/FITC FITC標記的羊抗小鼠IgM 0.3ml
Y149148 Goat Anti-Mouse IgM/RBITC 羅丹明標記的羊抗小鼠IgM 0.3ml
Y149149 Goat Anti-Mouse IgG/HRP 辣根過氧化物酶標記的羊抗小鼠IgG 0.1ml
Y149150 Goat Anti-Mouse IgG/Gold 膠體金標記的羊抗小鼠IgG 0.5ml
Y149151 Goat Anti-Mouse IgG/FITC FITC標記的羊抗小鼠IgG 0.3ml
Y149152 Goat Anti-Mouse IgG/RBITC 羅丹明標記的羊抗小鼠IgG 0.3ml
Y149153 Goat Anti-Mouse IgG/AP 堿性磷酸酶(AP)標記的羊抗小鼠IgG 0.1ml
Y149154 Goat Anti-Mouse IgG/PE-Cy7 PE-Cy7標記的羊抗小鼠IgG 0.1ml
Y149155 Goat Anti-Mouse IgG/PE-Cy5.5 PE-Cy5.5標記的羊抗小鼠IgG 0.1ml
Y149156 Goat Anti-Mouse IgG/Alexa Fluor 647 Alexa Fluor 647標記的羊抗小鼠IgG 0.1ml
Y149157 Goat Anti-Mouse IgG/Alexa Fluor 555 Alexa Fluor 555標記的羊抗小鼠IgG 0.1ml
Y149158 Goat Anti-Mouse IgG/Alexa Fluor 488 Alexa Fluor 488標記的羊抗小鼠IgG 0.1ml
Y149159 Goat Anti-Mouse IgG/Alexa Fluor 350 Alexa Fluor 350標記的羊抗小鼠IgG 0.1ml
Y149160 Goat Anti-Mouse IgG/APC APC標記的羊抗小鼠IgG 0.1ml
Y149161 Goat Anti-Mouse IgG/PE-CY5 PE-CY5標記的羊抗小鼠IgG 0.1ml
Y149162 Goat Anti-Mouse IgG/PE-Cy3 PE-Cy3標記的羊抗小鼠IgG 0.1ml
Y149163 Goat Anti-Mouse IgG/PE PE標記的羊抗小鼠IgG 0.1ml
Y149164 Goat Anti-Mouse IgG/Cy7 Cy7標記的羊抗小鼠IgG 0.1ml
Y149165 Goat Anti-Mouse IgG/Cy5.5 Cy5.5標記的羊抗小鼠IgG 0.1ml
Y149166 Goat Anti-Mouse IgG/Cy5 Cy5標記的羊抗小鼠IgG 0.1ml
Y149167 Goat Anti-Mouse IgG/Cy3 Cy3標記的羊抗小鼠IgG 0.1ml
Y149168 Goat Anti-Mouse IgG/Bio *標記的羊抗小鼠IgG 0.1ml
Y149169 Goat Anti-Bovine IgG/Bio *標記的羊抗牛IgG 0.1ml
Y149170 Goat Anti-Bovine IgG/HRP 辣根過氧化物酶標記的羊抗牛IgG 0.1ml
Y149171 Goat Anti-Bovine IgG/Cy5.5 Cy5.5標記的羊抗牛IgG 0.1ml
Y149172 Goat Anti-Bovine IgG/AP 堿性磷酸酶(AP)標記的羊抗牛IgG 0.1ml
Y149173 Goat Anti-Bovine IgG/Gold 膠體金標記的羊抗牛IgG 0.5ml
Y149174 Goat Anti-Bovine IgG/FITC FITC標記的羊抗牛IgG 0.3ml
Y149175 Goat Anti-Bovine IgG/RBITC 羅丹明標記的羊抗牛IgG 0.3ml
Y149176 Goat Anti-Bovine IgG/PE-Cy7 PE-Cy7標記的羊抗牛IgG 0.1ml
Y149177 Goat Anti-Bovine IgG/PE-Cy5.5 PE-Cy5.5標記的羊抗牛IgG 0.1ml
Y149178 Goat Anti-Bovine IgG/Alexa Fluor 647 Alexa Fluor 647標記的羊抗牛IgG 0.1ml
Y149179 Goat Anti-Bovine IgG/Alexa Fluor 555 Alexa Fluor 555標記的羊抗牛IgG 0.1ml
Y149180 Goat Anti-Bovine IgG/Alexa Fluor 488 Alexa Fluor 488標記的羊抗牛IgG 0.1ml
Y149181 Goat Anti-Bovine IgG/Alexa Fluor 350 Alexa Fluor 350標記的羊抗牛IgG 0.1ml
Y149182 Goat Anti-Bovine IgG/APC APC標記的羊抗牛IgG 0.1ml
Y149183 Goat Anti-Bovine IgG/PE-CY5 PE-CY5標記的羊抗牛IgG 0.1ml
Y149184 Goat Anti-Bovine IgG/PE-Cy3 PE-Cy3標記的羊抗牛IgG 0.1ml
Y149185 Goat Anti-Bovine IgG/PE PE標記的羊抗牛IgG 0.1ml
Y149186 Goat Anti-Bovine IgG/Cy7 Cy7標記的羊抗牛IgG 0.1ml
Y149187 Goat Anti-Bovine IgG/Cy5 Cy5標記的羊抗牛IgG 0.1ml
Y149188 Goat Anti-Bovine IgG/Cy3 Cy3標記的羊抗牛IgG大鼠Smad7 檢測試劑盒0.1ml
Y149189 Rabbit Anti-horse IgM/Alexa Fluor 647 Alexa Fluor 647標記的兔抗馬IgM 0.1ml
Y149190 Rabbit Anti-horse IgM/PE-Cy7 PE-Cy7標記的兔抗馬IgM 0.1ml
Y149191 Rabbit Anti-horse IgM/PE-Cy5.5 PE-Cy5.5標記的兔抗馬IgM 0.1ml
為了獲得更理想的ELISA實驗結果,請您
1、在ELISA實驗過程中,請精確配制標準品及工作液A和B,以避免由于不準確稀釋而造成的濃度誤差;
2、在ELISA實驗過程中,酶標板在溫育時應加膜覆蓋以避免液體蒸發;
3、在大鼠Smad7 檢測試劑盒實驗過程中,檢測液A和檢測液B,以及底物溶液在使用前,應置于37℃溫育30分鐘待用;
4、在ELISA實驗過程中,洗板后應盡快進行下一步操作,避免酶標板長時間處于干燥狀態。
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