詳細介紹
Littman 瓊脂
英文名稱:Littman Agar
多種培養基的配方
培養基的用途很廣,可以自己配,也可以買成品的
下面公布一下常用的培養基的配方,供大家參考使用
THE COMPOSITION OF MEDIA
培養基及成分
Acetobacter Medium (醋酸菌培養基)
Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g
CaCO3 20g Agar (瓊脂) 15g
Distilled water (蒸餾水) 1000ml Adjust (調) pH to 6.8
適用范圍:惡臭醋酸桿菌混濁變種
Lactic-bacteria Midium Ⅱ (乳酸菌培養基 Ⅱ)
Lacto-casein peptone (乳酪蛋白胨) 10g Beef extract (蛋白胨) 10g
Yeast extract (酵母膏) 5g Glucose (葡萄糖) 5g
Tween (吐溫) 80 1g K2HPO4 2g
Na-acetate (醋酸鈉) 5g Diamine citrate (檸檬酸二胺) 2g
MgSO4.7H2O 0.2g MnSO4.H2O 0.05g
Distilled water (蒸餾水) 1000m pH 6.5-6.8
適用范圍:植物乳桿菌(胚芽乳桿菌)
Peotone Glucose Yeast extract Medium PGY (蛋白胨、酵母膏、葡萄糖培養基)
Peptone(蛋白胨) 10g Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 1g Distilled water (蒸餾水) 1L
8. Glycerol Agar (甘油瓊脂)
Peptone (蛋白胨) 5g Beef extract (酵母膏) 3g
Glycerol (甘油) 20g Top water (自來水) 1000ml
Rhizobium medium (根瘤菌培養基)AS 9
Yeast eztract (酵母膏) 1g Soil eztract (土壤浸提液) 200ml
Mannitol (甘露醇) 10g Agar (瓊脂) 15g
Distilled water (蒸餾水) 800ml pH 7.2
[Note]:Soil extract:Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃ , then mixed with othringredients and distributed. (土壤浸提液的制法:取土壤50克,加水200毫升,15磅蒸煮1小時,經濾紙過濾后加水補足到200毫升。)
適用范圍:大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、
大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌