猴(Monkey)乙肝表面抗體（HBsAb）ELISA 檢測(cè)試劑盒
本試劑僅供研究使用 標(biāo)本：血清
試驗(yàn)原理：
HBsAb試劑盒是間接法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知HBsAb濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè)。先將HBsAb和*標(biāo)記的抗體同時(shí)溫育。洗滌后，加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌，去除未結(jié)合的酶結(jié)合物，然后加入底物A、B，和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中HBsAb的濃度呈比例關(guān)系。
猴(Monkey)乙肝表面抗體（HBsAb）ELISA 檢測(cè)試劑盒REAGENTS PROVIDED AND RECONSTTTUTION
REAGENTS（Store at 2-8℃） 1×96 WELLS 0.5×96 WELLS RECONSTTTUTION
96/48-wells microtiter plates 1 0.5 Ready-to-use
Plastiv cover 2 1 Ready-to-use
Standard: 800IU/L 1Vials (0.6ml) 0.5Vials (0.3ml) See reagents preparation on page 3
Blank control 1Vials (1.0ml) 1Vials (0.5ml) Ready-to-use
Standard Diluent 1Vials (5ml) 1Vials (2.5ml) Ready-to-use
Biotinylated anti-HBsAb 1Vials (6ml) 1Vials (3.0ml) Ready-to-use
Streptavidin-HRP 1Vials (10ml) 1Vials (5.0ml) Ready-to-use
Washing Buffer 1Vials (20ml) 1Vials (10ml) 50× concentrate
Substrate A 1Vials (6.0ml) 1Vials (3.0ml) Ready-to-use
Substrate B 1Vials （6.0ml) 1Vials (3.0ml) Ready-to-use
Stopping Solution 1Vials (6.0ml) 1Vials (3.0ml) Ready-to-use
MATERIAL REQUIRED BUT NOT PROVIDED
Distilled water
Pipettes:10ul、50ul、100ul、200ul、1000ul。
Vortex mixer and magnetic stirrer.
猴(Monkey)乙肝表面抗體（HBsAb）ELISA 檢測(cè)試劑盒SAFETY
For research use only
Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly water.
Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
Do not pipette by mouth.
PROCEDURAL NOTES/LAB.QUALITY CONTROL
When not in use, kit components should be stored refrigerated or frozen as indicated on vials or bottles. All reagents should be warmed to room temperature before use. Lyophilized standards should be discarded after use.
Once the desired number of strips has been removed, immediay reseal the bag to protect the remaining strips from edterioration.
Cover or cap all reagents when not in use.
Do not mis or interchange reagents between different lots.
Do not use reagents beyond the expiration date of the kit .
Use a clean disposable plastic pipette tip for each reagent, standard, or specimen addition in order to avoid cross-contamination, for the dispensing of H2SO4 and substrate solution, avoid pipettes with metal parts.
Use a clean plastic container to prepare the washing solution.
Thoroughly mix the reagents and samples before use by agitation or swirling.
All residual washing liquid must be drained from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells.
The TMB solution is light sensitive. Avoid prolonged exposure to light, also, avoid contact of the TMB solution with metal to prevent colour development. Warning TMB is toxic avoid direct contact with hands. Dispose off properly. If a dark blue colour develops within a few minutes after preparation, this indicates that the TMB solution has been contaminated and must be discarede. Read absorbances within 1 hour after completion of the assay.
When pipetting reagents, maintain a consistent order of addition from well-to-well. This will ensure equal incubation times for all wells.
Respect incubation times described in the assay procedure.
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