Rabbit Anti-IGF1R/CD61抗體說明書

Cat. Number:  RS-0342R

Quantity size:  0.1ml  （dilute with pH 7.4    0.01 M PBS or diluent of antibody）

Background: This receptor binds insulin-like growth factor 1 （IGF1） with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.

Specificity:      ·anti- CD61 is a rabbit monoclonal antibody unconjugated

                ·specific for CD61 of   rat and mo 

                ·epitope mapping near the amino terminus of CD61 of  

                   hu, rat and mo origin

                · use for western blotting, elisa, immunoprecipitation    

                   and immunohistochemistry

·  Protein A affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt: 82kDa

Application:     · Western blotting: 1:100-500

· Immunohistochemistry: 1:100-500

· ELISA: 1:500-1000

·  Optimal working dilutions must be determined by the end user.

Storage:   Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.

Important Note:  This product as supplied is  intended for research use only, not for use in human, therapeutic or diagnostic applications.

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