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Anti-CK18/FITC
Catalog Number : RSF-1059R-FITC
Quantity size : 100ul (2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA and 0.1% Sodium azide. )
Background: Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
Specificity : · anti- CK18 is a mouse monoclonal antibody, /FITC
· specific for CK18 of Human
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt:48kDa
Application : .IF=1:50-200
· Immunohistochemistry : 1:20-1:200
· Immunocytochemistry: 1:20-1:200
· Excitation spectrum: 490-495nm
· Emission spectrum: 520-530nm
. KLH conjugated synthetic peptide derived from human CK18 (53-96aa)
· Optimal working dilutions must be determined by the end user.
· protect from light.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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