中文名稱    細胞毒性T細胞抗原-4抗體    
別    名    CD 152; CD152; CD152 antigen; Celiac disease 3; CELIAC3; CTLA 4; CTLA-4; Cytotoxic T cell associated 4; Cytotoxic T lymphocyte associated 4; Cytotoxic T lymphocyte associated antigen 4; Cytotoxic T lymphocyte associated protein 4; Cytotoxic T lymphocyte associated serine esterase 4; Cytotoxic T lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; Cytotoxic T-lymphocyte-associated protein 4; GSE; IDDM12.      
Specific References  (1)     |     bs-10006R has been referenced in 1 publications.
[IF=4.12] Seike, Masahiro, et al. "Histamine suppresses regulatory T cells mediated by TGF‐β in murine chronic allergic contact dermatitis." Experimental Dermatology(2015).  IHC-F ;  Mouse.  
PubMed:25651189
研究領域    
抗體來源    Rabbit    
克隆類型    Polyclonal    
交叉反應    Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep,     
產品應用    WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg/Test  
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    
分 子 量    21kDa    
細胞定位    細胞膜 細胞外基質     
性    狀    Lyophilized or Liquid    
濃    度    1mg/ml    
免 疫 原    KLH conjugated synthetic peptide derived from human CTLA-4/CD152:75-170/223 <Extracellular>    
亞    型    IgG    
純化方法    affinity purified by Protein A    
儲 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    
保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    
PubMed    PubMed    
產品介紹    background:
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
抗原抗體結合特點
 1）特異性：抗原分子只能與由它刺激所產生的抗體結合而起反應的專一性能；
 2）可逆性：是指Ag與相應Ab結合成IC后，在一定條件下可解離為游離抗原與抗體。
 3）階段性：
 一階段：特異性結合階段 （①反應速度快，幾秒鐘或幾分鐘即可完成 ②不出現肉眼可見反應）；
 第二階段：可見階段（表現為凝集、沉淀、補體結合等反應 ①反應進行慢，需要幾分鐘、幾十分鐘或geng長②受電解質、溫度、酸堿度等多種因素影響）。
4）比例性：（zui適比或等價點）是指抗原與抗體發生可見反應需遵循一定的量比關系，只有當二者濃度比例適當時，才出現可見反應。
抗原抗體反應影響因素：
（一）反應物自身的因素
5） 抗體：不同來源的抗體,反應性各有差異,抗體的濃度、特異性和親和力都影響抗體抗原反應,為提高試驗的可靠性,應選擇高特異性、高親和力的抗體作診斷試劑.等價帶的寬窄也影響抗原抗體復合物的形成,單克隆抗體不適用于沉淀反應.
6）  抗原：抗原的理化性狀、分子量、抗原決定簇的種類及數目均可影響反應結果.顆粒性抗原出現凝集反應,可溶性抗原出現沉淀反應,單價抗原與相應抗體結合不出現沉淀現象.
（二）反應環境條件
7） 酸堿度：抗原抗體反應必須在合適的pH環境中進行.蛋白質具有兩性電離性質,因此每種蛋白質都有固定的等電點.抗原抗體反應一般在pH6～9進行,有補體參與的反應pH為7.7.4,pH過高或過低都將影響抗原與抗體反應.
8）  溫度：在一定范圍內,溫度升高可加速分子運動,抗原與抗體碰撞機會增多,使反應加速.一般為15℃～40℃,常用的抗原抗體反應溫度為37℃,溫度如高于56℃,可導致已結合的抗原抗體再解離,甚至變性或破壞.每種試驗都有其*的zui適反應溫度要求.此外,適當振蕩也可促進抗原抗體分子的接觸,加速反應.