產品名稱：細胞表面趨化因子受體6抗體，CCR-6/CD196抗體
　　相關標記： HRP  Biotin  Gold  RBITC  AP  FITC  Cy3  Cy5  Cy5.5  Cy7  PE  PE-Cy3  PE-CY5  PE-CY5.5  PE-CY7  APC  Alexa Fluor 350  Alexa Fluor 488  Alexa Fluor 555 Alexa Fluor 647
　　濃 度： 1mg/1ml
　　抗體來源： Rabbit or Mouse
　　克隆類型： polyclonal or monoclonal
　　產品類型 一抗
　　研究領域 腫瘤 信號轉導 轉錄調節因子 細胞表面分子
　　蛋白分子量 predicted molecular weight: 41kDa
　　性 狀 Lyophilized or Liquid
　　免 疫 原 KLH conjugated synthetic peptide derived from human CCR6 C-terminus
　　亞 型 IgG
　　純化方法 affinity purified by Protein A
　　儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
　　產品應用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

　　保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
　　Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

　　產品介紹 CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein coupled receptors. It is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha （MIP 3 alpha）. This receptor has been shown to be important for B lineage maturation and antigen driven B cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. CCR6 has been reported to be expressed in intestine, colon, liver, lymph node, spleen, lung, blood, umbilical cord, placenta, skin, and pancreas. It has also been reported to be expressed in lymphocytes, T cells, and B cells.

　　免疫組化結果分析：
　　1、首先要確定免疫組化技術是否合格，合格的染色結果應該是背景顏色淺淡或無著色，而陽性標志物清晰和定位明確。
　　切片中的正常組織可以作為-簡便的對照來判斷染色結果的可靠與否，例如用Vimentin抗體，間質成纖維細胞利血管壁平滑肌細胞胞漿應呈陽性。肯定為陽性的對照組織卻呈陰性，待測組織的染色結果也就不可靠；這現象常是由于抗體失效或過度稀釋、組織中抗原嚴重丟失等。
　　2、不僅要判斷所檢組織中有或無陽性標記物，還要注意陽性信號的分布部位和形態特征。
　　一般說來，陽性信號應與被測抗原所在的部位一致，大部分細胞抗原位于胞質內，其中亦有區別，有的陽性顆粒彌散于整個細胞的胞質，有的則呈斑塊狀局限于胞漿某處。還有些抗原同時分布于胞質和胞膜(如胃腸腺癌細胞的EMA)，或核與胞質(如S-100、HMB-45)。同一種抗原在不同類型的細胞可定位不同，因而分布的特點也有一定的鑒別意義。