【產(chǎn)品名稱】：膜輔蛋白抗體，Anti-CD46/MCP抗體

【產(chǎn)品類型】：一抗

【相關(guān)標(biāo)記】: HRP Biotin Gold RBITC AP FITC Cy3 Cy5 Cy5.5 Cy7 PE PE-Cy3 PE-CY5 PE-CY5.5 PE-CY7 APC Alexa Fluor 350 Alexa Fluor 488 Alexa Fluor 555 Alexa Fluor 647

【性 狀】： Lyophilized or Liquid

【濃 度】： 1mg/1ml

【亞 型】： IgG

【規(guī) 格】：0.1ml/100ug，0.2ml/200ug

【抗體來(lái)源】： Rabbit OR MOUSE

【克隆類型】： polyclonal or monoclonal

【產(chǎn)品用途】：科研實(shí)驗(yàn)，用于免疫組化實(shí)驗(yàn)，WB實(shí)驗(yàn)、IF、IP、ELISA實(shí)驗(yàn)，相應(yīng)的標(biāo)記抗體有HRP標(biāo)記抗體，F(xiàn)ITC標(biāo)記，BIO等。

【貯 存】： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

【詳細(xì)介紹】：CD46 is one of the membrane complement regulatory proteins. CD46 possesses C3b-binding and factor I cofactor activities which play important roles in the regulation of the complement activation pathway. CD46 is widely distributed on blood cells, endothelial cells, epithelial cells and tumor cell lines. CD46 exists as many isoforms in a variety of tissues. The antigen has a broad distribution and is present on leukocytes, plaets, endothelial cells, epithelial cells and fibroblasts. It is strongly expressed on salivary gland ducts and kidney ducts, moderay on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. It is expressed on thymocytes, B cells, monocytes, granulocytes, NK cells, plaets, epithelial and endothelial cells.

免疫組化結(jié)果分析：

1、首先要確定免疫組化技術(shù)是否合格，合格的染色結(jié)果應(yīng)該是背景顏色淺淡或無(wú)著色，而陽(yáng)性標(biāo)志物清晰和定位明確。

切片中的正常組織可以作為-簡(jiǎn)便的對(duì)照來(lái)判斷染色結(jié)果的可靠與否，例如用Vimentin抗體，間質(zhì)成纖維細(xì)胞利血管壁平滑肌細(xì)胞胞漿應(yīng)呈陽(yáng)性。肯定為陽(yáng)性的對(duì)照組織卻呈陰性，待測(cè)組織的染色結(jié)果也就不可靠；這現(xiàn)象常是由于抗體失效或過(guò)度稀釋、組織中抗原嚴(yán)重丟失等。

2、不僅要判斷所檢組織中有或無(wú)陽(yáng)性標(biāo)記物，還要注意陽(yáng)性信號(hào)的分布部位和形態(tài)特征。

一般說(shuō)來(lái)，陽(yáng)性信號(hào)應(yīng)與被測(cè)抗原所在的部位一致，大部分細(xì)胞抗原位于胞質(zhì)內(nèi)，其中亦有區(qū)別，有的陽(yáng)性顆粒彌散于整個(gè)細(xì)胞的胞質(zhì)，有的則呈斑塊狀局限于胞漿某處。還有些抗原同時(shí)分布于胞質(zhì)和胞膜(如胃腸腺癌細(xì)胞的EMA)，或核與胞質(zhì)(如S-100、HMB-45)。同一種抗原在不同類型的細(xì)胞可定位不同，因而分布的特點(diǎn)也有一定的鑒別意義。