當前位置:上海邦景實業有限公司>>科研抗體>>免疫血清>> 羊抗牛IgG免疫血清抗體
羊抗牛IgG免疫血清抗體本公司提供的熒光抗體是將抗血清,經純化成抗體IgG成分,采用直接標記方法,將異硫氰酸熒光素(FITC美國sigma產品)及異硫氰酸羅達明熒光素(紅色)(RBITC, sigma產)共價交聯到抗體(IgG)分子的賴氨酸分子上,并經過純化、蛋白量、FITC、RBITC濃度及效價鑒定而制成。
羊抗牛IgG免疫血清抗體應用范圍:
1.用于細菌、病毒、梅毒螺旋體、鉤端螺旋體等微生物的抗原或抗體檢查。
2.用于臨床自身免疫病的多種自身抗體熒光檢查,如抗核抗體,抗心肌抗體等。
3.細胞因子等檢查的熒光流式細胞儀測定。
4.白細胞分型抗原的免疫熒光染色。
5.免疫組織及免疫病理中抗原,抗體檢查,如各種腫瘤抗原檢查。
主要性能:每支0.5ml液體,含有0.03%NaN3防腐,IgG蛋白,濃度在5mg左右,FITC為sigma異構體I,約在70?80μg/ml,495nm為zui大吸收峰。RBITC約20?40μg/mlzui大吸收555nm,zui大熒光光譜波長為600nm。工作濃度用0.01mol/LpH7.4PBS作1:10?1:20稀釋使用,-20℃可保存一年,盡量少反復凍融。通稱第二抗體。
保存條件: -20°C
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