FITC-CM-polysucroses are standard FITC-polysucrose carrying carboxymethyl-substituents. Polysucrose (renamed from Ficoll™) is a polymer which displays excellent biocompatibility. The CM (carboxymethyl) group gives the product anionic (negative) charge. All batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. TdB Labs produce FITC-CM-polysucroses with a molecular weight of 70 kDa and 400 kDa. FITC-CM-polysucrose is supplied as a yellow powder.

Structure

Polysucrose is a polymer synthesised by cross-linking sucrose with epichlorohydrin. FITC-CM-polysucroses are manufactured by reacting selected polysucrose fractions with an activated carboxymethyl derivative in alkali whereby O-carboxymethyl groups are introduced along the polysucrose chain. The carboxyl content is approximately 5% which is equivalent to about one CM group for every five glucose units. Thereafter, fluorescein (FITC) groups are introduced by reaction with fluorescein isothiocyanate. Polysucrose has a more spherical structure and is less flexible compared to dextran.

Spectral data

FITC-polysucrose has an excitation maximum of 495 and an emission maximum at 517 nm at pH 9.

Storage and stability

FITC-CM-polysucrose is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature.

Solubility

FITC-CM-polysucrose dissolves readily in water.

Application

FITC-CM-polysucrose are mostly used for studies of permeability and microcirculation. The carboxyl groups will impart an overall negative charge to the molecule, which may be valuable in gaining information on the permeability characteristics of cell membranes and tissues. FITC-CM-polysucrose has played an interesting role in elucidating the properties of the glomerular membrane.

艾美捷CM-polysucrose（CM-聚蔗糖，羧甲基-聚蔗糖，Carboxymethyl-polysucrose）#CMP70：

FITC-CM-聚蔗糖是攜帶羧甲基取代基的標準FITC-聚蔗糖。聚蔗糖（從Ficoll™更名）是一種具有優(yōu)異生物相容性的聚合物。CM（羧甲基）基團賦予產(chǎn)物陰離子（負）電荷。檢查所有批次的分子量、取代度、干燥失重和游離 FITC。TdB實驗室生產(chǎn)分子量為70 kDa和400 kDa的FITC-CM-聚蔗糖。FITC-CM-聚蔗糖以黃色粉末形式提供。

結(jié)構(gòu)：

聚蔗糖是通過蔗糖與環(huán)氧氯丙烷交聯(lián)合成的聚合物。FITC-CM-聚蔗糖是通過將選定的聚蔗糖級分與堿中的活化羧甲基衍生物反應來制造的，從而沿聚蔗糖鏈引入O-羧甲基。羧基含量約為5%，相當于每五個葡萄糖單位約一個CM基團。此后，通過與異硫氰酸熒光素反應引入熒光素（FITC）基團。與葡聚糖相比，聚蔗糖具有更球形的結(jié)構(gòu)，并且靈活性較差。

光譜數(shù)據(jù)：

FITC-聚蔗糖在pH 495時的激發(fā)最大值為517，在9 nm處的發(fā)射最大值。

儲存和穩(wěn)定性

FITC-CM-polysucrose在環(huán)境溫度下干燥儲存在密封良好的容器中時可穩(wěn)定保存 6 年以上。

溶解性

FITC-CM-polysucrose易溶于水。

FITC-CM-polysucrose的應用：

主要用于滲透性和微循環(huán)的研究。羧基將賦予分子整體負電荷，這對于獲得有關(guān)細胞膜和組織的通透性特征的信息可能是有價值的。FITC-CM-聚蔗糖在闡明腎小球膜的性質(zhì)方面發(fā)揮了有趣的作用。